Homogeneous mammalian hematopoietic stem cell composition

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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435 2, 43524025, 424 8591, 424577, 436808, A01N 102, C12N 502, C12P 2100, A61K 3900

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050875701

ABSTRACT:
Highly concentrated hematopoietic stem cell compositions are provided which are substantially free of differentiated or dedicated hematopoietic cells. The cells are obtained by subtraction of cells having particular markers and selection of cells having particular markers. The resulting composition may be used to provide for individual or groups of hematopoietic lineages, to reconstitute stem cells of the host, and to identify an assay for a wide variety of hematopoietic growth factors.

REFERENCES:
patent: 4714680 (1987-12-01), Civin
Whitlock and Witte, 1982. PNAS 79:3608-3612, Long-term Culture of B Lymphocytes & Their Precursors . . . .
Basch et al., Cell Separation Using Positive Imnunoselective Techniques J. Immun. Methods 56:200 1983.
Stites et al. Basic & Clinical Immun. 5th Ed. p. 765.
Muller-Sieburg et al. 1986, Cell 44:653-662, Isolation of Two Early B-Lymphocyte Progenitors . . . .

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