Homogeneous immunoassays using enzyme inhibitors

Details Homogeneous immunoassays using enzyme inhibitors Homogeneous immunoassays using enzyme inhibitors

1995-05-26

1999-07-06

Huff, Sheela

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 71, G01N 3353

Patent

active

059196416

ABSTRACT:
A method for determining the presence of an analyte which is a specific binding pair member in a sample suspected of containing the analyte is described. The method involves: bringing together in an aqueous medium, the sample, an enzyme bound to a first specific binding pair member and an inhibitor for the enzyme bound to a second specific binding pair member wherein each specific binding pair member is capable of binding to the analyte or to a specific binding pair member complementary to the analyte; analyzing the medium for the activity of said enzyme; and relating the activity to the amount of analyte present in the medium. Compositions of matter and kits are also described.

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