Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals
Patent
1981-10-13
1983-12-20
Marantz, Sidney
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
2504581, 356318, 356417, 436519, 436520, 436527, 436532, 436533, 436534, 436537, 436800, 436807, G01N 3354, G01N 3358, G01N 3352, G01N 2164
Patent
active
044218605
ABSTRACT:
In the course of a reaction in which one of the reactants is on the surface of carrier particles in a solution and another of the reactants is tagged with a fluorescent substance, some of the fluorescently tagged reactant attaches to, or is displaced from the carrier particle. The present invention relates to a method and device for determining the amount of fluorescently-tagged reactant which is attached to the carrier particle or which is free in solution, without physically separating the carrier particles from the solution. In a particular application of the invention (immunoassay) the reaction is between antibodies and antigens, and from the amount of fluorescently-tagged reactant which is attached to the carrier particle one can determine the unknown amount of antigen in a sample. The number of fluorescent particles which are bound to the large carrier particles and which are in solution are determined by optical and electronic sensing and analyzing techniques, including (1) shadowing techniques in which large opaque carrier particles block the pick-up of fluorescent radiation from bound tagged reactants, and/or (2) autocorrelation techniques which selectively discriminate between radiation from the bound fluorescently tagged reactants on the large particles and the free fluorescently-tagged reactants which have a more rapid diffusion movement than the carrier particles.
REFERENCES:
patent: 4275149 (1981-06-01), Litman
patent: 4318707 (1982-03-01), Litman
Elings Virgil B.
Nicoli David F.
Marantz Sidney
The Regents of the University of California
LandOfFree
Homogeneous fluoroimmunoassay involving autocorrelation processi does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Homogeneous fluoroimmunoassay involving autocorrelation processi, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Homogeneous fluoroimmunoassay involving autocorrelation processi will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-108165