Homogeneous fluorescence ligang binding assay based upon prefere

Details Homogeneous fluorescence ligang binding assay based upon prefere Homogeneous fluorescence ligang binding assay based upon prefere

1983-08-01

1987-02-03

Warren, Charles F.

Chemistry: analytical and immunological testing

Involving producing or treating antigen or hapten

Producing labeled antigens

436501, 436537, 436800, 436826, 436829, G01N 33533

Patent

active

046408983

ABSTRACT:
Fluorescence ligand binding assay of a sample containing an unknown amount of ligand may be performed by making direct intensity measurements. In an immunoassay, for example, the sample may be added to a solution containing fluorescein labeled analyte and then is added an antibody specific to the analyte. Sodium dodecyl sulfate, a surfactant, added to the solution in an amount sufficient to form micelles provides markedly different fluorescent intensity from bound and unbound labeled analyte.

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