Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1983-12-14
1986-12-16
Marantz, Sidney
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 18, 435 25, 435 27, 435 28, 435175, 435810, 436501, 436518, G01N 33543
Patent
active
046296905
ABSTRACT:
Enzyme channeling assay is provided employing a solid non-porous surface to which is bound a member of a specific binding pair and an enzyme. A complementary member of a specific binding pair is conjugated to second enzyme, so that the amount of second enzyme which becomes bound to said solid non-porous surface by the binding of a specific binding pair(s) is related to the amount of analyte in the liquid assay medium with which the solid surface is in contact. The first and second enzymes are related by the product of one being the substrate of the other. The turnover of the substrate formed by one of the enzymes results in a detectable product, which can be related to the amount of analyte in the medium.
REFERENCES:
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patent: 4233402 (1980-11-01), Maggio et al.
patent: 4275149 (1981-06-01), Litman et al.
patent: 4299916 (1981-11-01), Litman et al.
patent: 4391904 (1983-07-01), Litman et al.
Gibbons Ian
Ullman Edwin F.
Weng Litai
Leitereg Theodore J.
Marantz Sidney
Rowland Bertram I.
Saunders David A.
Syntex (U.S.A.) Inc.
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