Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1982-08-18
1985-04-09
Marantz, Sidney
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
436536, 436537, G01N 3354
Patent
active
045102406
ABSTRACT:
A process for the determination of an antigen or hapten in homogeneous aqueous phase by incubation in the presence of antigen- or hapten-specific antibodies and of a definite amount of enzyme-marked antigen or hapten and measurement of the activity of the marker enzyme, wherein, after incubation, the reaction solution is heated under those temperature conditions and for a period of time at which the marker enzyme is inactivated by at least 50% in the absence of the antigen- or hapten-specific antibody and the enzyme activity thereafter measured.
Also a reagent for carrying out this process, wherein it contains a definite amount of .beta.-galactosidase-hapten conjugate, hapten antibody, buffer (pH 6.0 to 8.5), a system for the determination of the .beta.-galactosidase activity and optionally a definite amount of .beta.-galactosidase antibody.
REFERENCES:
patent: 4341866 (1982-07-01), Yoshida
"Enzyme-Immunoassay", E. T. Maggio, ed., Chapt. 5, pp. 105-134, by E. F. Ullman et al., CRC Press, Boca Raton, (1980).
Boehringer Mannheim GmbH
Marantz Sidney
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