Homogeneous enzyme immunoassay system and method

Details Homogeneous enzyme immunoassay system and method Homogeneous enzyme immunoassay system and method

1986-08-20

1988-11-08

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 14, 435 26, 435177, 435188, 435810, 436528, 436535, 436815, 436816, 436821, 436829, G01N 3353

Patent

active

047834005

ABSTRACT:
A liposome assay reagent for determination of an analyte in a homogeneous immunoassay. The reagent includes a suspension of oligolamellar lipid vesicles containing encapsulated glucose-6-phosphate dehydrogenase (G6PD), at a specific activity of between about 1-15 units/.mu.mole vesicle lipid, and glucose-6-phosphate (G6P) at a concentration of at least about 5 mM. The encapsulated G6P protects the enzyme against inactivation on preparation, by reverse phase evaporation in the presence of organic solvent, and on storage as an aqueous suspension.

REFERENCES:
patent: 4322311 (1982-03-01), Lim et al.
patent: 4338094 (1982-07-01), Elahi
patent: 4342826 (1982-08-01), Cole
patent: 4473638 (1984-09-01), Auditore-Hargreaves
patent: 4532089 (1985-07-01), MacDonald
patent: 4581222 (1986-04-01), Baldeschwieler et al.
patent: 4622294 (1986-11-01), Kung et al.

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