Homogeneous enzyme immunoassay for ferritin

Details Homogeneous enzyme immunoassay for ferritin Homogeneous enzyme immunoassay for ferritin

1983-09-23

1985-12-24

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435188, 435810, 436532, 436801, G01N 5300, G01N 33549, C12N 996

Patent

active

045606480

ABSTRACT:
Composition and method are provided for enzyme immunoassays for high molecular weight proteins, such as ferritin. An enzyme is conjugated to the high molecular weight protein through a specific linking group. Usually, on the average, about 2 to 10 enzyme molecules are bonded to each protein molecule. In an assay, the conjugate competes with an unknown sample for receptor and the resulting enzymatic activity is compared to a standard for a determination of the presence and amount of protein in the unknown.

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