Homogeneous DNA probe titration assay

Details Homogeneous DNA probe titration assay Homogeneous DNA probe titration assay

1995-12-19

1998-05-05

Campbell, Eggerton A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, C12Q 168, C12P 1924

Patent

active

057472564

ABSTRACT:
An assay for detecting the presence or amount of a target polynucleotide sequence of interest in a test sample is disclosed. The assay comprises the steps of forming a reaction mixture by combining in an assay medium (i) a first reagent and (ii) an aliquot of the test sample suspected of containing the target polynucleotide sequence. The reaction mixture is subjected to denaturing conditions, and exposed to hybridization conditions allowing the first reagent to hybridize with any target polynucleotide present. A second reagent is then added, the reaction mixture undergoes hybridization conditions and the change in turbidity of the reaction mixture is detected, correlating with the presence of the target polynucleotide in the sample.

REFERENCES:
patent: 4045384 (1977-08-01), Dorman
patent: 4275149 (1981-06-01), Litman
patent: 4925785 (1990-05-01), Wang et al.
patent: 5026785 (1991-06-01), Mage
patent: 5306615 (1994-04-01), Breillatt, Jr.
patent: 5306619 (1994-04-01), Edwards
Polsky-cynkin, R.; Clinical Chemistry: Use of DNA immobilized on plastic and Agarose Supports to Detect DNA by Sandwich Hybridization, pp. 1449 (vol. 31, No. 9).
Paul, W.E.; Fundamental Immunology: Hemagglutination and Hemaggultination Inhibition, 1984, pp. 638-640.

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