Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-07-25
1999-01-12
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 41, 935 76, 935 77, 935 78, C12Q 168, C12P 100, C12N 1500
Patent
active
058586652
ABSTRACT:
The present invention relates to a method, kit and reagent composition for determining the presence of a target nucleic acid in a sample using a two-stage target cycling reaction. During stage 1 of the reaction, the target nucleic acid hybridizes with a probe nucleic acid to effectuate release of an activator into the assay medium. During stage 2 of the reaction, a target analog-anchor complex is cleaved by the activator, which accomplishes the dual purpose of releasing the target analog into the assay medium, and the initiation of signal generation. The released target analog then restarts the cyclic reaction by binding to a second probe, which effectuates release of a second activator, and so on. This cyclic reaction gives rise to an increase in the amount of signal generated from a single target nucleic acid molecule in the sample, which greatly enhances the level of target detection that can be expected.
REFERENCES:
patent: 4463090 (1984-07-01), Harris
patent: 4699876 (1987-10-01), Libeskind
patent: 5011769 (1991-04-01), Duck et al.
patent: 5118605 (1992-06-01), Urdea
patent: 5190864 (1993-03-01), Giese et al.
patent: 5288611 (1994-02-01), Kohne
patent: 5352775 (1994-10-01), Albertsen et al.
patent: 5445942 (1995-08-01), Rabin et al.
patent: 5462871 (1995-10-01), Boon-Falleur et al.
patent: 5470723 (1995-11-01), Walker et al.
patent: 5494796 (1996-02-01), Spears et al.
patent: 5500341 (1996-03-01), Spears
patent: 5508168 (1996-04-01), Orle et al.
patent: 5512444 (1996-04-01), Patard et al.
patent: 5518884 (1996-05-01), Spears et al.
patent: 5527676 (1996-06-01), Vogestein et al.
patent: 5529780 (1996-06-01), Paoletti et al.
patent: 5532108 (1996-07-01), Vogelstein
patent: 5534438 (1996-07-01), Hayden et al.
patent: 5536636 (1996-07-01), Freeman, Jr. et al.
patent: 5536638 (1996-07-01), Rossau et al.
Southern, "Detection of specific sequences among DNA fragments separated by gel electrophoresis" J. Mol. Biol. (1975) 98:503-517.
Nelson et al., "Detection of acridinium esters by chemiluminescence" Nonisotopic DNA Probe Techniques, (1992) Academic Press, New York, pp. 275-310.
Saiki et al., "Primer-dircted enzymatic amplification of DNA with a thermostable DNA polymerase" Science (1988) 239:487-491.
Wu et al., "The ligation amplification reaction (LAR)-amplification of specific DNA sequences using sequential rounds of template-dependent ligation" Genomics (1990) 4: 560-569.
Urdea et al., "Synthesis and characterization of branched DNA (bDNA) for the direct and quantitive detection of CMV, HBV, HCV, and HIV" Clin. Chem. (1993) 39:725-726.
Bekkaoui et al., "Cycling probe technology with RNase H attached to an oligonucleotide" BioTechniques (1996) 20:240-248.
Sambrook et al., Molecular Cloning: A Laboratory Manual, Second Edition (1989) Cold Spring Harbor Laboratory Press, Chapter 11, pp. 11.1-11.61.
Stec et al., "Automated solid-phase synthesis, separation, and stereochemistry of phosphorothioate analogues of oligdeoxyribonucleotides" J. Am. Chem. Soc. (1984) 106:6077-6079.
Wong, Chemistry of Protein Conjugation and Cross-Linking (1991) CRC Press, Inc. Boca Raton, Florida. The title page and table of contents are enclosed herewith.
Hermanson, Bioconjugate Techniques (1995) Academic Press, New York, pp. 649-651.
Sambrook et al., Molecular Cloning, A Laboratory Manual, Second Edition (1989) Cold Spring Harbor Laboratory Press, Chapter 5, pp. 5.1-5.95.
Stevens et al., "Real time detection of quantitive PCR using multiple fluorogenic probes" Clinical Chemistry (1995) 41:1683. (Abstract 022).
Gingeras et al., "Hybridization properties of immunobilized nucleic acids" Nucleic Acids Res. (1987) 15:5373-5390.
Hermanson, Bioconjugate Techniques (1990) Academic Press, New York, Section 3.2 pp. 55-56.
Lund et al., "Assessment of methods for covalent binding of nucleic acids to magnetic heads, Dynabeds.TM., and the characteristics of the bound nucleic acids in hybridization reactions" Nucl. Acids Res. (1988) 16:10861-10880.
Yamashina, "The action of enterokinase on trypsinogen" Biochim. Biophys. Acta. (1956) 20:433-434.
Hermanson, Bioconjugate Techniques (1996) Academic Press, New York, Section 2.4 pp. 662-664.
Magee et al., "Specific one-stage method for assay of enterokinase activity H!acetyl-trypsinogen and the effect of calcium ions on the enzyme activity" Biochem J. (1981) 197:239-244.
Product Brochure, "CFLPScan.TM. Kits: Sensitive detection of mutations in minutes" Boehringer Mannheim Biochemicals (Mar. 1996), 3 pages total.
Mifflin et al., "Use and diagnostic applications of the polymerase chain reaction (PCR)" Workshop for Forty-Seventh National Meeting, American Association for Clinical Chemistry, Jul. 18, 1995, Anaheim, CA, 30 page total.
Betsch, "Nucleic acid amplification-based diagnostics: Barriers to commercialization" IVD Technology (Jan. 1995) pp. 22-28.
Compton, "Nuclic acid sequence-based amplification" Nature (1991) 350:91-92.
Dirksen et al., "Selective inhibition of RNase H by dextran" J. Biol. Chem. (1981) 256:11569-11573.
Hofstee, "Soluble stoichiometric complexes of DNA with chymotrypsin and trypsin" Biochim. Biophys. Acta. (1960) 44:194-195.
Houston et al., "The transient inactivation of trypsin by mild acetylation with N-acetylimidazole" Biochemistry (1970) 9:156-166.
Nilsson et al., "Thin-layer immunoaffinity chromatography with bar code quantitation of C-reactive protein" Anal. Chem. (1995) 67:3051-3056.
Owens et al., "Sensitive and rapid diagnosis of patato spindle tuber viroid disease by nucleic acid hybridization" Science (1981) 213:670-672.
Radding, "Helical interactions in homologous pairing and strand exchange driven by RecA protein" J. Biol. Chem. (1991) 9:5355-5358.
Robinson et al., "The relation of the .alpha.-amino group of trypsin to enzyme function and zymogen activation" Biochemistry (1973) 12:420-425.
Vratsanos et al., "On the mechanism of enzyme action. LXVII. Acetylation of trypsin in organic solvents" Archives of Biochemistry and Biophysics (1953) 77:216-226.
Walker, "Inducible DNA repair systems" Ann. Rev. Biochem. (1985) 54:425-457.
West et al., "recA protein promotes homologous-pairing and strand-exchange reactions between duplex DNA molecules" Proc. Natl. Acad. Sci. USA (1981) 78:2100-2104.
Wang, "The profile advantage" Profile Diagnostic Sciences, Inc., 510 East 73rd Street, New York, NY 10021, 14 pages total.
Product Brochure, "Faster Mutation Analysis" Ambion, Inc., 2130 Woodward Street, #200, Austin, TX 78744-1832, 6 pages total.
Matthews et al., Analytical Biochemistry 169 :1-25 (1988).
Kulisek et al., Analytical Biochemistry 177 : 78-84 (1989).
Zimmerman et al., Analytic Biochemistry 78 :47-51(1977).
Hepp Jozsef
Lengyel Zsolt
Pande Rajiv
Jones W. Gary
Navix, Inc.
Whisenant Ethan
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