Homogeneous detection of a target through nucleic acid ligand-li

Details Homogeneous detection of a target through nucleic acid ligand-li Homogeneous detection of a target through nucleic acid ligand-li

1998-09-18

1999-11-23

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 71, 536 243, 536 231, C12Q 168, C07H 2104

Patent

active

059898232

ABSTRACT:
The presence of a target in a test mixture or the concentration of such target can be determined by a method relying on fluorescence emission measurement. Such method utilizes a nucleic acid ligand to the target and a ligand beacon comprising: i) a nucleic acid sequence complimentary to at least a portion of said nucleic acid ligand, ii) a fluorescent group, and iii) a fluorescence-modifying group. The emission profile of the ligand beacon is altered based on the presence and/or concentration change of the target in the test mixture.

REFERENCES:
patent: 5118801 (1992-06-01), Lizardi et al.
patent: 5270163 (1993-12-01), Gold et al.
patent: 5312728 (1994-05-01), Lizardi et al.
patent: 5691145 (1997-11-01), Pitner et al.
patent: 5723323 (1998-03-01), Kauffman et al.
Tyagi et al., Nature Biotechnology 14, 303-308.
Ellington & Szostak (1990) Abstracts of papers presented at the 1990 meeting on RNA Processing, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, p. 84.
Joyce (1989) Gene 82:83.
Joyce & Inoue (1989) Nucleic Acids Research 17:711.
Kinzler & Vogelstein (1989) Nucleic Acids Research 17:3645.
Kostrikis et al. (1998) Science 279:1228.
Kramer et al. (1974) J. Mol. Biol. 89:719.
Levisohn & Spiegelman (1969) Proc. Natl. Acad. Sci. USA 63:805.
Levisohn & Spiegelman (1968) Proc. Natl. Acad. Sci. USA 60:866.
Nyren et al. (1993) Analytical Biochem. 208:171.
Oliphant et al. (1989) Mol. Cell. Biol. 9:2944.
Oliphant & Struhl (1988) Nucleic Acids Research 16:7673.
Oliphant & Struhl (1987) Methods in Enzymology 155:568.
Oliphant et al. (1986) Gene 44:177.
Robertson & Joyce (1990) Nature 344:467.
Thiesen & Bach (1990) Nucleic Acids Research 18:3203.
Tyagi et al. (1998) Nature Biotechnology 16:49.

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