Homogeneous denatured human 06-guanine alkyltransferase prepared

Details Homogeneous denatured human 06-guanine alkyltransferase prepared Homogeneous denatured human 06-guanine alkyltransferase prepared

1993-05-11

1994-05-31

Naff, David M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

435803, 435814, 530413, C12N 910, C07K 300

Patent

active

053169328

ABSTRACT:
A denatured human O.sup.6 -guanine alkyltransferase is disclosed. The enzyme is prepared by a process which involves denaturing the enzyme, contacting the denatured enzyme with a monoclonal antibody specific for the denatured enzyme on a substrate to which the monoclonal antibody is bound to, so that the denatured enzyme and the monoclonal antibody form an immunocomplex and then, eluting the denatured enzyme from the substrate-bound monoclonal antibody so that the denatured human O.sup.6 -guanine alkyltransferase is obtained. The enzyme can be used to develop probes for the Mer.sup.- phenotype and these probes in turn are contemplated for use in identifying drug resistant tumors in patients.

REFERENCES:
patent: 4123427 (1978-10-01), Daniel
patent: 4474893 (1984-10-01), Reading
patent: 4789631 (1988-12-01), Maggio
patent: 4789734 (1988-12-01), Pierschbacher
patent: 5136027 (1992-08-01), Pope
patent: 5158875 (1992-10-01), Miller et al.
patent: 5162507 (1992-11-01), Wolfe et al.
patent: 5175104 (1992-12-01), Asahi et al.
patent: 5187080 (1993-02-01), Andrews e tal.
patent: 5187153 (1993-02-01), Cordell et al.
patent: 5191063 (1993-03-01), Inouye et al.
Harris et al., Cancer Research, vol. 43, 3247-3252, 1983.
Dolan et al., Carcinogenesis (London), 9 (11), Sequence Specificity of Guanine . . . , pp. 2139-2143, 1988.
Wiestler et al., Carcinogenesis (London), 5 (1), O.sup.6 -Alkylguanine-DNA alkytransferase . . . , pp. 121-124, 1984.
Hall et al., Carcinogenesis (London), 6 (2), O.sup.6 -Alkylguanine-DNA alkyltransferase . . . , 207-211, 1985.
Grafstrom et al., Cancer Research, 44, O.sup.6 -Alkylguanine-DNA Alkyltransferase Acitivity . . . , pp. 2855-2857, 1984.
Geysen et al., Science, vol. 235, Mar. 6, 1987, pp. 1184-1190.
Sharma et al., Proc. Nat'l. Acad. Sci. U.S.A., vol. 77, No. 10, pp. 5865-5868, 1980.
Jones, Amer. J. Physiol. S2: 203-207, 1920.
Sela et al., Biochima FT Biophysica, Act A, 26:S02-512, 1957.

Affiliated with

Also associated with

Rating

Homogeneous denatured human 06-guanine alkyltransferase prepared does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Homogeneous denatured human 06-guanine alkyltransferase prepared, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Homogeneous denatured human 06-guanine alkyltransferase prepared will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1627507