Homogeneous binding assay

Details Homogeneous binding assay Homogeneous binding assay

1997-01-23

1999-08-17

Housel, James C.

Chemical apparatus and process disinfecting, deodorizing, preser

Analyzer, structured indicator, or manipulative laboratory...

Calorimeter

422 50, 422 55, 422 41, 422 8205, 422 8209, 436518, 436501, 436525, 436527, 436805, 436808, 4352831, 435 6, 4352872, 435975, G01N 2100, G01N 33543, C12M 100, A01N 102

Patent

active

059390212

ABSTRACT:
A homogeneous method of measuring chemical binding relies on resonant, or "amplified," optical extinction (light scattering plus absorption) from a defined, specific class of colloidal particles wherein the real term n of the complex refractive index n-ik approaches zero while the imaginary term k approaches .sqroot.2. Chemical binding partners are coated onto the particles, which either aggregate or disperse during the binding reaction, causing an optical extinction change at one wavelength that is quantitatively related to the number of single colloidal particles and another at a second wavelength that is quantitatively related to the number of doublet colloidal particles. The method employs: (1) a specific class of colloidal particles that exhibit optical resonance and that are substantially smaller in diameter than a wavelength of visible light; (2) a one-step process of colloidal particle aggregation; and (3) a photometric extinction measurement at wavelengths where the particles exhibit optical resonance.

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