Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1990-08-06
1993-05-11
Moskowitz, Margaret
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 18, 435 91, 435196, 435805, 436501, 436 63, 436815, 536 243, 935 17, 935 77, 935 78, 935 88, C12Q 168, C07H 2100
Patent
active
052100154
ABSTRACT:
The present invention is directed to a process of detecting a target nucleic acid using labeled oligonucleotides. This process uses the 5' to 3' nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification assay.
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Lehman et al. (1973) Journal of Biological Chemistry, vol. 248, No. 22, pp. 7717-7723.
Gelfand David H.
Holland Pamela M.
Saiki Randall K.
Watson Robert M.
Gould George M.
Hoffman-La Roche Inc.
Marschel Ardin H.
Moskowitz Margaret
Sias Stacev R.
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