Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals
Patent
1989-06-09
1993-03-30
Saunders, David
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
435817, 436537, 436538, 436544, 436806, 436817, 436818, 530363, 530807, 530812, G01N 33536, G01N 33543
Patent
active
051983672
ABSTRACT:
A method is described for measuring the amount of analyte present in a sample containing the analyte using a homogeneous amperometric immunoassay. The analyte is covalently bonded to a suitable carrier molecule, which is also covalently bonded to an electroactive molecule. The electroactive molecule, such as ferrocene carboxylic acid, contains a redox center which is capable of transferring a charge to an electrode. A preferred carrier molecule is bovine serum albumin (BSA), while suitable analytes include digoxin, theophylline and HCG. The immunoassay is conveniently performed by applying a voltage to a set of electrodes.
REFERENCES:
patent: 4233144 (1980-11-01), Pace et al.
patent: 4882013 (1989-11-01), Turner et al.
patent: 5053497 (1991-10-01), Hwang et al.
Heineman et al., Analytical Chemistry 57(12) pp. 1321-1331 (1985).
Chemical Abstracts 105(15) p. 354 (No. 130350m) (Oct. 13, 1986) (note JP-A-61100660 (May 1986)).
Mizutani et al., "Ferrocene-attached bovine serum albumin as a mediator between glucose oxidase and an electrode", Denki Kagaku oyobi Kogyo Bitsuri Kagaku, 1988, vol. 56, pp. 1100-1101, (Abstract).
Aizawa Masuo
Hidaka Miki
Manning Brenda D.
Uretsky Laura S.
Chin Christopher L.
Morgenstern Arthur S.
Saunders David
Slepchuk, Jr. Nicholas I.
LandOfFree
Homogeneous amperometric immunoassay does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Homogeneous amperometric immunoassay, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Homogeneous amperometric immunoassay will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1280624