Homogeneous amperometric immunoassay

Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals

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435817, 436537, 436538, 436544, 436806, 436817, 436818, 530363, 530807, 530812, G01N 33536, G01N 33543

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active

053468325

ABSTRACT:
A method is described for measuring the amount of analyte present in a sample containing the analyte using a homogeneous amperometric immunoassay. The analyte is chemically bonded to a suitable carrier molecule, which is also chemically bonded to an electroactive molecule. The electroactive molecule, such as ferrocene carboxylic acid, contains a redox center which is capable of transferring a charge to an electrode. A preferred carrier molecule is bovine serum albumin (BSA), while suitable analytes include digoxin, theophylline and HCG. The immunoassay is conveniently performed by applying a voltage to a set of electrodes.

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patent: 5053497 (1991-10-01), Hwang et al.
patent: 5198367 (1993-03-01), Aizawa et al.
Mizutani et al, "Ferrocene attached bovine serum albumin as a mediator between glucose oxidase and an electrode", Denki Kagaku oyobi Kogyo Bitsuri Kagaku, 1988, vol. 56, pp. 1100-1101.
Heineman et al, Analytical Chemistry 57(12) pp. 1321-1331 (1985).
Chemical Abstracts 105(15) p. 354 (No. 130350m) (Oct. 13, 1986) (note JP-A-61100660 (May 1986).

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