Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals
Patent
1992-12-18
1994-09-13
Scheiner, Toni R.
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
435817, 436537, 436538, 436544, 436806, 436817, 436818, 530363, 530807, 530812, G01N 33536, G01N 33543
Patent
active
053468325
ABSTRACT:
A method is described for measuring the amount of analyte present in a sample containing the analyte using a homogeneous amperometric immunoassay. The analyte is chemically bonded to a suitable carrier molecule, which is also chemically bonded to an electroactive molecule. The electroactive molecule, such as ferrocene carboxylic acid, contains a redox center which is capable of transferring a charge to an electrode. A preferred carrier molecule is bovine serum albumin (BSA), while suitable analytes include digoxin, theophylline and HCG. The immunoassay is conveniently performed by applying a voltage to a set of electrodes.
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Mizutani et al, "Ferrocene attached bovine serum albumin as a mediator between glucose oxidase and an electrode", Denki Kagaku oyobi Kogyo Bitsuri Kagaku, 1988, vol. 56, pp. 1100-1101.
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Aizawa Masuo
Hidaka Miki
Manning Brenda D.
Uretsky Laura S.
Chin Christopher L.
Ciba Corning Diagnostics Corp.
Morgenstern Arthur S.
Roesler Judith A.
Scheiner Toni R.
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