Homocysteine assay

Details Homocysteine assay Homocysteine assay

1998-06-18

2000-02-01

Warden, Jill

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

Amino acid or sequencing procedure

422 61, G01N 3368

Patent

active

060202068

ABSTRACT:
This invention pertains to methods of determining the presence and/or quantity of homocysteine in a sample containing other thiol-containing compounds. The methods of this invention involve modifying the homocysteine to facilitate the separation of homocysteine from cysteine. An assay protocol comprises adjusting the conditions of the sample suspected of containing homocysteine so that homocysteine forms homocysteine thiolactone, separating the homocysteine thiolactone from free thiol-containing compounds present in the sample, including cysteine, reconverting the homocysteine thiolactone to homocysteine, and determining the presence and/or quantity of homocysteine in the sample by conventional means.

REFERENCES:
patent: 4571430 (1986-02-01), Bryne et al.
patent: 4940658 (1990-07-01), Allen et al.
patent: 5274122 (1993-12-01), Tolman et al.
patent: 5346599 (1994-09-01), Stamler et al.
patent: 5438017 (1995-08-01), Allen et al.
patent: 5478729 (1995-12-01), Van Atta et al.
patent: 5559038 (1996-09-01), Kolhouse et al.
patent: 5631127 (1997-05-01), Sundrehagen
patent: 5668173 (1997-09-01), Garrow
Andersson et al. (1992) European Journal of Clinical Investigation 22:79-87 month unknown.
Andersson et al. (1993) Clin. Chem. 39:1590-1597 month unknown.
Araki and Sako (1987) Journal of Chromatography 422:43-52 month unknown.
Fahey et al. (1981) Analytical Biochemistry 111:357-365 month unknown.
Fiskerstrand et al. (1993) Clin. Chem. 39:263-271 month unknown.
Graham et al. (1997) JAMA 277:1775-1781 month unknown.
Jacobsen et al. (1989) Analytical Biochemistry 178:208-214 month unknown.
Kang et al. (1982) Pediatr. Res. 16:370-372 month unknown.
Mansoor et al. (1992) Analytical Biochemistry 200:218-229 month unknown.
Racker (1955) J. Biol. Chem. 217:867-875 month unknown.
Refsum et al. (1985) Clin. Chem. 31:624-628 month unknown.
Refsum et al. (1989) Clin. Chem. 35:1921-1927 month unknown.
Stabler et al. (1987) Analytical Biochemistry 162:185-196 month unknown.
Stern et al. (1982) Journal of Biochemical and Biophysical Methods 7:83-89 month unknown.
Ueland et al. (1993) Clin. Chem. 39:1764-1779 month unknown.
Velury and Howell (1988) Journal of Chromatography 424:141-146 month unknown.

Affiliated with

Also associated with

Rating

Homocysteine assay does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Homocysteine assay, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Homocysteine assay will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-936819