Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2004-12-15
2009-06-02
Kam, Chih-Min (Department: 1656)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S325000, C435S007720, C536S023100, C530S300000
Reexamination Certificate
active
07541143
ABSTRACT:
The present invention provides for novel reagents whose fluorescence changes upon cleavage or a change in conformation of a backbone. The reagents comprise a backbone (e.g. nucleic acid, polypeptide, etc.) joining two fluorophores of the same species whereby the fluorophores form an H-dimer resulting in quenching of the fluorescence of the fluorophores. When the backbone is cleaved or changes conformation, the fluorophores are separated, no longer forming an H-type dimer, and are de-quenched thereby providing a detectable signal. The use of a single fluorophore rather than an “acceptor-donor” fluoresecence resonance energy transfer system offers synthesis and performance advantages.
REFERENCES:
patent: 4557862 (1985-12-01), Mangel et al.
patent: 4648893 (1987-03-01), Roux
patent: 4708929 (1987-11-01), Henderson
patent: 4780421 (1988-10-01), Kameda et al.
patent: 4897444 (1990-01-01), Brynes et al.
patent: 5011910 (1991-04-01), Marshall et al.
patent: 5110801 (1992-05-01), Leveen et al.
patent: 5118801 (1992-06-01), Lizardi et al.
patent: 5212298 (1993-05-01), Rademacher et al.
patent: 5312728 (1994-05-01), Lizardi et al.
patent: 5506115 (1996-04-01), Toth et al.
patent: 5539082 (1996-07-01), Nielsen et al.
patent: 5605809 (1997-02-01), Komoriya et al.
patent: 5652107 (1997-07-01), Lizardi et al.
patent: 5698411 (1997-12-01), Lucas et al.
patent: 5714342 (1998-02-01), Komoriya et al.
patent: 5723288 (1998-03-01), Dykstra et al.
patent: 5733719 (1998-03-01), Jaffe et al.
patent: 5759773 (1998-06-01), Tyagi et al.
patent: 5776720 (1998-07-01), Jaffe et al.
patent: 5804395 (1998-09-01), Schade et al.
patent: 5807674 (1998-09-01), Tyagi et al.
patent: 5871946 (1999-02-01), Lucas et al.
patent: 5912137 (1999-06-01), Tsien et al.
patent: 5925517 (1999-07-01), Tyagi et al.
patent: 5925558 (1999-07-01), Tsien et al.
patent: 5998204 (1999-12-01), Tsien et al.
patent: 6037130 (2000-03-01), Tyagi et al.
patent: 6037137 (2000-03-01), Komoriya et al.
patent: 6103476 (2000-08-01), Tyagi et al.
patent: 6150097 (2000-11-01), Tyagi et al.
patent: 6410255 (2002-06-01), Pollok et al.
patent: WO 96/13607 (1996-05-01), None
patent: WO 97/39008 (1997-10-01), None
patent: WO 98/10096 (1998-03-01), None
patent: WO 98/37226 (1998-08-01), None
patent: WO 00/06778 (2000-02-01), None
patent: WO 00/71562 (2000-11-01), None
patent: WO 00/71740 (2000-11-01), None
patent: WO 01/18238 (2001-03-01), None
patent: 01/31062 (2001-05-01), None
Holland et al., PNAS 88, 7276-7280 (1991).
Knight et al. “A novel coumarin-labelled peptide for sensitive continuous assats of the matrix metalloproteinases.”FEBBS Letters296(3):263-266 (1992).
Carmel et al. “Use of Substrates with Fluorescent Donor and Acceptor Chromophores for the Kinetic Assay of Hydrolases.”FEBBS Letters30(1):11-14 (1973),
Isaac et al. “Use of Flurescence Resonance Energy Transfer to Estimate Intramolecular Distances in the Msx-1 Homeodomain.”Biochemistry34(1):15276-15281 (1995).
Keller et al. “Mode of Insertion of the Signal Sequence of a Bacterial Precusor Protein into Phospholipid Bilayers As Revealed by Cysteine-Based Site Directed Spectroscopy.”Biochemistry35:3063-3071 (1996).
Ekert et al.EMBO J. 18(2):330-338 (1999).
Finucane et al.Abstract J. Biol. Chem274(4):2225-2233 (1999).
Harvey et al.Jol. Of Cell Biol. 148(1) (2000), p. 57-92.
Hirata et al.J. of Exp. Med. 187(4) (1998), p. 587-600.
Kanuka et al.PNAS USA96:145-150 (1999).
KomoriyaCambridge Health Inst. (2001).
Komoriya et al.J. of Exp. Mel191(11):1819-1829 (2000).
Packard et al.Abratacts 40th Annual Meeting(1996).
Packard et al.Biophysical Chemistry 67167-176 (1997).
Packard et al.J. Phys. Chem B. 101:5070-5074 (1997).
Packard et al.J. Phys. Chem B752-758 (1998).
Packard et al.J. Phys. Chem. B1820-1827 (1998).
Packard et al.Methods in Enzymology278:15-23 (1997).
Packard et al.PNAS USA93:11640-11645 (1996).
Perez et al.Molecular Human Reproduction5(5):414-420 (1999).
Robels et al.Endocrinology(1999) Abstract, vol. 140, No. 6, p. 2641-2644.
Siegel et al.Jol. Of Call Ciol. 141(5) (1998).
Zapata et al.Jol. Of Biol Chem. 273(12):6916-6920 (1998).
Bernacchi and Mely (2001) Nucleic Acids. Res., 29(13): e62.
Sokol et al. (1998) Proc. Natl. Acad. Scil, USA, 95: 11538-11543.
Latt et al. “Flourescence Determination of Carboxypeptidase A Activity Based on Electronic Energy Transfer.”Analytical Biochemistry50:56 (1972).
Matayoshi et al. “Novel Fluorogenic Substrates for Assaying Retroviral Proteases by Resonance Energy Transfer.”Science247 (1990).
Matsuzaki et al. “Translocation of a Channel-Forming Antimicrobial Peptide, Maganin 2, across Lipid Bilayers by Forming a Pore.”Biochemistry34:6521-6526 (1995).
Nagase et al. “Design and Characterization of a Fluorogenic Substrate Selectively Hydrolyzed by Stromelysin 1 (Matrix Metalloproteinase-3)”J. of Biol. Chem269:20952 (1994).
Packard et al. “Intramolecular Excitonic Dimers in Protease Substrated: Modification of the Backbone Moiety to Probe the H-Dimer Structure.”American Chem Society1-8 (1998).
Parkhurst et al. “Donor-Accoptor Distance Distributions in a Double-Labeled Fluorescent Oligonucleotide Both as a Single Strand and in Duplexes.”Biochemistry34(1):293-300, (1995).
Parkhurst et al. Kinetic Studies by Fluorescence Resonance Energy Transfer Employing a Double-Labeked Oligonucleotide: Hybridization to the Oligonucleotide Complement and to Single Strand DNABiochemistry34:285-292 (1995).
Wang et al. “Design and Synthesis of New Flourogenic HIV Protease Substrates Based on Resonance Energy Transfer.”Tetrahedron Letters31:6493 (1990).
Wu et al. “Resonance Energy Transfer: Methods and Applications.”Analytical Biochemistry218:1-13 (1994).
Yang et al. “Conformational Flexibality of Three-Way DNA Junctions Containing Inpaired Nucleotides.”Biochemistry35:7959-7967 (1996).
Komoriya Akira
Packard Beverly
Hunter Tom
Kam Chih-Min
OnCoimmunin, Inc.
Weaver Austin Villeneuve & Sampson LLP
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