Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of...
Reexamination Certificate
2005-05-17
2005-05-17
Weber, Jon (Department: 1653)
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
C435S023000, C435S007720, C436S172000, C514S002600, C530S300000
Reexamination Certificate
active
06893868
ABSTRACT:
The present invention provides for novel reagents whose fluorescence changes upon cleavage or a change in conformation of a backbone. The reagents comprise a backbone (e.g. nucleic acid, polypeptide, etc.) joining two fluorophores of the same species whereby the fluorophores form an H-dimer resulting in quenching of the fluorescence of the fluorophores. When the backbone is cleaved or changes conformation, the fluorophores are separated, no longer forming an H-type dimer, and are de-quenched thereby providing a detectable signal. The use of a single fluorophore rather than an “acceptor-donor” fluoresecence resonance energy transfer system offers synthesis and performance advantages.
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Komoriya Akira
Packard Beverly
Kam Chih-Min
Onco Immunin, Inc.
Quine I.P. Law Group. P.C.
Weber Jon
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