Homing endonuclease which originates from chlamydomonas eugameto

Details Homing endonuclease which originates from chlamydomonas eugameto Homing endonuclease which originates from chlamydomonas eugameto

1992-12-16

1995-05-30

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

4352576, C12N 922

Patent

active

054200326

ABSTRACT:
The present invention relates to a homing endonuclease which originates from Chlamydomonas eugametos, and was overproduced in E. coli, purified and characterized. The homing endonuclease of the present invention recognizes and cleaves degenerate double-stranded DNA at a specific recognition site; it particularly recognizes and cleaves 15, 17 and 19 nucleotide sequences. The cleavage of target DNA by this endonuclease produces a 4 nucleotide extension with a 3' OH overhang. A method to use the endonuclease of the present invention to cleave DNA fragments useful for gene mapping is also disclosed.

REFERENCES:
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