Drug – bio-affecting and body treating compositions – In vivo diagnosis or in vivo testing – Testing efficacy or toxicity of a compound or composition
Reexamination Certificate
2005-10-18
2005-10-18
Falk, Anne-Marie (Department: 1632)
Drug, bio-affecting and body treating compositions
In vivo diagnosis or in vivo testing
Testing efficacy or toxicity of a compound or composition
C424S009100, C435S325000, C435S366000, C435S368000, C435S377000
Reexamination Certificate
active
06955802
ABSTRACT:
Provided are genetically engineered cells comprising a neural stem cell and retroviral expression system in the neural stem cell, which is capable of expressing homeodomain transcription factor Nkx6.1 protein but does not express homeodomain transcription factor Irx3 protein or homeodomain transcription factor Nkx2.2 protein; which is capable of expressing homeodomain transcription factor Nkx6.1 protein and homeodomain transcription factor Irx3 protein; and which is capable of expressing homeodomain transcription factor Nkx2.2 protein or homeodomain transcription factor Nkx2.9 protein. Also provided are methods of generating such genetically engineered motor neurons, V2 neurons, and V3 neurons. Also provided are methods of treating subjects having a motor neuron injury or a motor neuron disease comprising implanting in injured/diseased neural tissue of the subject any of the provided genetically engineered cells, administering to such neural tissue retroviral expression systems which are capable of expressing the appropriate homeodomain protein(s), or transfecting neural stem cells with a retroviral vector, which is capable of expressing the required homeodomain transcription factor protein(s). Provided is a method of determining whether a chemical compound affects the generation of a motor neuron from a neural stem cell.
REFERENCES:
patent: 6235885 (2001-05-01), Jessell et al.
patent: 6387656 (2002-05-01), Jessell et al.
patent: 6566092 (2003-05-01), Jessell et al.
patent: 2002/0197678 (2002-12-01), Jessell et al.
patent: 2003/0104374 (2003-06-01), Jessell et al.
patent: 2004/0005602 (2004-01-01), Jessell et al.
patent: WO 95/23223 (1995-08-01), None
patent: WO 99/00516 (1999-01-01), None
patent: WO00/09676 (2000-02-01), None
patent: WO 00/18884 (2000-04-01), None
patent: WO0184933 (2001-11-01), None
patent: WO0218545 (2002-03-01), None
Horner et al. Regenerating the damaged central nervous system vol. 407 pp. 963-970 2000.
Jackowski Neural Injury repair: hope for the future as barriers to effective CNS regeneration become clearer pp. 303-317 1995.
Hamburger, V. et al. (1951) “A Series Of Normal Stages In The Development Of The Chick Embryo”J. Morphol.88:49-92 (Exhibit 1).
Langman, J. et al. (1966) “Behavior of Neuroepithelial Cells During Closure Of The Neural Tube”J. Comp. Neur.127:399-411 (Exhibit 2).
Leber, S.M. et al. (1995) “Migratory Paths Of Neurons And Glia In The Embryonic Chick Spinal Cord”J. Neurosci.15:1236-1248 (Exhibit 3).
Muramatsu, T. et al. (1997) “Comparison Of Three Nonviral Transfection Methods For Foreign Gene Expression In Early Chicken Embryons In Ovo”Biochem. Biophys. Res. Commun.230:376-380 (Exhibit 4).
Sander, M. et al. (2000) “Ventral Neural Patterning By Nkx Homeobox Genes: Nkx6.1 Controls Somatic Motor Neuron And Ventral Interneuron Fates”Genes&Development14(17):2134-2139 (Exhibit 5).
Struhl, G. et al. (1992) “Control Of Drosophila Body Pattern By The hunchback Morphogen Gradient”Cell69:237-249 (Exhibit 6); and.
Yamada, T. et al. (1993) “Control Of Cell Pattern In The Neural Tube: Motor Neuron Induction By Diffusible Factors From Notochord And Floor Plate”Cell73:673-686 (Exhibit 7).
Cai, J. et al. (2001) “Mice lacking theNkx6.2(Gtx) homeodomain transcription factor develop and reproduce normally,”Molecular and Cellular Biology21:4399-4403.
Palmer, T.D. et al. (1999) “Fibroblast growth factor-2 activates a latent neurogenic program in neural stem cells from diverse regions of the adult CNS,”The Journal of Neuroscience19: 8487-8497.
Briscoe, J. et al., (1999) “Homeobox Gene Nkx2.2 and specification of Neuronal Identity by Graded Sonic Hedgehog Signalling”Nature398:622-627 (Exhibit 1).
Campbell, G. et al., (1999) “Transducing the Dpp Morphogen Gradient in the Wing of Drosophila: Regulation of Dpp Targets by brinker”Cell96:553-562 (Exhibit 2).
Chiang, C. et al., (1996) “Cyclopia and Defective Axial Patterning in Mice Lacking Sonic Hedgehog Gene Function”Nature383:407-413 (Exhibit 3).
Dasen, J.S. et al., (1999) “Combinatorial Codes in Signaling and Synergy: Lessons From Pituitary Development”Curr Opin. Genet. & Dev.9:566-574 (Exhibit 4).
Ding, Q. et al., (1998) “Diminished Sonic Hedgehog Signaling and Lack of Floor Plate Differentiation in Gli2 Mutant Mice”Development125:2533-2543 (Exhibit 5).
Doetsch, F. et al., (1999) “Subventricular Zone Astrocytes Are Neural Stem Cells in the Adult Mammalian Brain”Cell97:703-716 (Exhibit 6).
Ericson, J. et al., (1996) “Two Critical Periods of Sonic Hedgehog Signaling Required for the Specification of Motor Neuron Identity”Cell87:661-673 (Exhibit 7).
Ericson, J. et al., (1997) “Pax6 Controls Progenitor Cell Identity and Neuronal Fate in Response to Graded Shh Signaling”Cell90:169-180 (Exhibit 8).
Ericson, J. et al., (1997) “Graded Sonic Hedgehog Signaling and the Specification fo Cell Fate in the Ventral Neural Tube”Cold Spring Harb. Symp. Quant. Biol.62:451-466 (Exhibit 9).
Erskine, L. et al., (1998) “Progenitor Dispersal and the Origin of Early Neuronal Phenotypes in the Chick Embryo Spinal Cord”Dev. Biol. 199:26-41 (Exhibit 10).
Funayama, N. et al., (1999) “Coelom Formation: Binary Decision of the Lateral Plate Mesoderm is Controlled by the Ectoderm”Development126:4129-4138 (Exhibit 11).
Gage, F.H. (2000) “Mammalian Neural Stem Cells”Science287:1433-1438 (Exhibit 12).
Huang, A.M. et al., (1997) “An Anteroposterior Dorsal Gradient in the Drosophila Embryo”Genes&Dev. 11:1963-1973 (Exhibit 13).
Ingham, P.W. (1998) “Transducing Hedgehog: The Story So Far”EMBO J. 17:3505-3511 (Exhibit 14).
Jazwinska, A. et al., (1999) “The Drosophila Gene brinker Reveals a Novel Mechanism of Dpp Target Gene Regulation”Cell96:563-573 (Exhibit 15).
Johansson, C.B. et al., (1999) “Identification of a Neural Stem Cell in the Adult Mammalian Central Nervous System”Cell96:25-34 (Exhibit 16).
Kraut, R. et al., (1991) “Spatial Regulation of the Gap Gene giant During Drosophila Development”Development111:601-609 (Exhibit 17).
Krishnan, V. et al., (1997) “Mediation of Sonic Hedgehog-Induced Expression of COUP-TFII by a Protein Phosphatase”Science278:1947-1950 (Exhibit 18).
Lawrence, P.A. et al., (1996) “Morphogens, Compartments, and Pattern: Lessons from Drosophila?”Cell85:951-961 (Exhibit 19).
Lewis, K.E. et al., (1999) “Expression of ptc and gli Genes in talpid3Suggests Bifurcation in Shh Pathway”Development126:2397-2407 (Exhibit 20).
Lumsden, A. et al., (1996) “Patterning the Vertebrate Neuraxis”Science274:1109-1115 (Exhibit 21).
Mansouri, A. et al., (1998) “Pax3 and Pax7 are Expressed in Commissural Neurons and Restrict Ventral Neuronal Identity in the Spinal Cord”Mech. Dev.78:171-178 (Exhibit 22).
Marti, E. et al., (1995) “Distribution of Sonic Hedgehog Peptides in the Developing Chick and Mouse Embryo”Development121:2537-2547 (Exhibit 23).
Matise, M.P. et al., (1998) “Gli2 is Required for Induction of Floor Plate and Adjacent Cells, But Not Most Ventral Neurons in the Mouse Central Nervous System”Development125:2759-2770 (Exhibit 24).
McDowell, N. et al., (1999) “Activin as a Morphogen in Xenopus Mesoderm Induction”Semin. Cell & Dev. Biol. 10:311-317 (Exhibit 25).
Minami, M. et al., (1999) “Brinker is a Target of Dpp in Drosophila that Negatively Regulates Dpp-dependent Genes”Nature398:242-246 (Exhibit 26).
Pabst, O. et al., (1998) “Nkx-9 is a Novel Homeobox Transcription Factor Which Demarcates Ventral Domains in the Developing Mouse CNS”Mech. Dev.73:85-93 (Exhibit 27).
Papin, C. et al., “Gradual Refinement fo Activin-Induced Thresholds Requires Protein Synthesis”Dev. Biol.217:166-172 (Exhibit 28).
Pierani,
Briscoe James
Ericson Johan
Jessell Thomas M.
Cooper & Dunham LLP
Falk Anne-Marie
The Trustees of Columbia University in the City of New York
White, Esq. John P.
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