Holographic microscopy of holographically trapped...

Optical: systems and elements – Holographic system or element – Using a hologram as an optical element

Reexamination Certificate

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C359S368000, C359S385000, C359S577000, C359S902000

Reexamination Certificate

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07839551

ABSTRACT:
A method and system for performing three-dimensional holographic microscopy of an optically trapped structure. The method and system use an inverted optical microscope, a laser source which generates a trapping laser beam wherein the laser beam is focused by an objective lens into a plurality of optical traps. The method and system also use a collimated laser at an imaging wavelength to illuminate the structure created by the optical traps. Imaging light scattered by the optically tapped structure forms holograms that are imaged by a video camera and analyzed by optical formalisms to determine light field to reconstruct 3-D images for analysis and evaluation.

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