Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1994-07-29
1997-09-23
McElwain, Elizabeth
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 691, 4352404, 4353201, 536 231, 536 241, 800205, C12N 1509, C12N 1582, A01H 500
Patent
active
056703490
ABSTRACT:
The invention relates in part to plant HMG2 HMGR genes and in part to the "post-harvest" production method of producing gene product of interest in plant tissues and cultures. The HMG2 promoter elements are responsive to pathogen-infection, pest-infestation, wounding, or elicitor or chemical treatments. The HMG2 elements are also active in specialized tissues of the plant including pollen and mature fruits. HMG2 promoter elements and HMG2-derived promoters can be advantageously used to drive the expression of disease and pest resistance genes, whereby transgenic plants having such gene constructs would be resistant to the targeted disease and pest. In particular, the HMG2 gene expression system can be utilized in developing nematode resistant plants. The post-harvest production method of the invention utilizes plant tissues and cell cultures of plants or plant cells engineered with a expression construct comprising an inducible promoter, such as the HMG2 promoter, operably linked to a gene of interest. Production of the desired gene product is obtained by harvesting, followed by inducing and processing the harvested tissue or culture. The post-harvest production method may be advantageously used to produce direct or indirect gene products that are labile, volatile, toxic, hazardous, etc.
REFERENCES:
Aoyagi et al., 1993, Plant Physiol. 102:622-638.
Bach et al., 1991, American Oil Chemists Society, Champaign, IL, pp. 29-49.
Bach et al., 1991, Lipids 26:637-648.
Brooker and Russell, 1975, Arch. Biochem. Biophys. 167:730-737.
Brooker and Russell, 1979, Arch. Biochem. Biophys. 198:323-334.
Caelles et al., 1989, Plant Mol. Biol. 13:627-638.
Chappell et al., 1991, Plant Physiol. 97:693-698.
Chappell et al., 1991, Plant Physiol. 96:127.
Choi et al., 1989, Phytopathology 79(10):1157.
Choi et al., 1992, Plant Cell 4:1333-1344.
Chye et al., 1991, Plant Mol. Biol. 16:567-577.
Chye et al., 1992, Plant Mol. Biol. 19:473-484.
Cramer et al., 1985, EMBO J. 4:285-289.
Cramer et al., 1985, Science, 227:1240-42.
Cramer et al., 1989, J. Cell. Biochem. 13D:M408.
Cramer et al., 1991, J. Cell Biochem Suppl (15 Part A, p. 54).
Cramer et al., 1993, Journal of Nematology 25:507-518.
Dixon et al., 1986, Philos Trans R Soc Lond B Biol Sci. 314:411-426.
Dixon and Harrison, 1990, Adv. Genet. 28:165-234.
Genschik et al., 1992, Plant Mol. Biol. 20:337-341.
Goddijn et al., 1993, The Plant Journal 4:863-873.
Ji et al., 1992, Physiol. Plant, 84(2):185-192.
Learned and Fink, 1989, Proc. Natl. Acad. Sci. USA 86:2779-2783.
Maldonado-Mendoza et al., 1992, Plant Physiol. 100:1613-1614.
Narita and Gruissem, 1989, Plant Cell 1:181-190.
Narita et al., 1991, J. Cell. Biochem 15A:102(Abst).
Nelson et al., 1991, Abstract 1322, 3rd Intl. Cong. Int. Soc. Plant Mol. Biol., Tucson, AZ.
Opperman et al., 1994, Science 263:221-223.
Park et al., 1989, Phytopathology 79(10):1198.
Park, Heesung (1990) Ph.D. Dissertation (complete), Virginia Polytechnic Institute and State University "Molecular Cloning, Characterization and Expression of 3-hydroxy-3-methylglutaryl CoenzymeA Reductase Gene From Tomato (Lycopersicon esculentum, Mill)".
Park et al., 1992, Plant Mol. Biol. 20:327-331.
Russell and Davidson, 1982, Biochem. Biophys. Res. Comm. 104:1537-1543.
Ryder et al., Organization, Structure and Activation of Plant Defense Genes, in Biology and Molecular Biology of Plant Pathogen Interactions, J. A. Bailey, ed., Springer-Verlag, (1986) pp. 207-219.
Shih and Kuc, 1973, Phytopathology 63:826-829.
Stermer and Bostock, 1987, Plant Physiol. 84:404-408.
Stermer et al., 1991, Physiol. Mol. Plant Pathol. 39:135-145.
Stermer et al., 1992, Phytopathology 82:1085.
Wong et al., 1982, Arch. Biochem. Biophys. 216:631-638.
Yang et al., 1989, Mol. Plant-Microb. Interact. 2:195-201.
Yang et al., 1989, Phytopathology 79(10):1150-51.
Yang et al., 1991, Plant Cell 3:397-405.
An et al., 1990, "Nopaline synthase promoter is wound inducible and auxin inducible", Plant Cell 2:225-233.
Arias et al., 1993, "Dissection of the functional architecture of plant defense gene promoter using a homologous in vitro transcription initiation system", Plant Cell 5:485-496.
Becker-Andre et al., 1991, "Structural comparison, modes of expression, and putative cis-acting elements of the two 4-coumarate:CoA ligase genes in potato", J. of Biol. Chem. 266:8551-8559.
Benhamou et al., 1991, "Accumulation of .beta.-Fructosidase in the cell walls of tomato roots following infection by a fungal wilt pathogen", Plant Physiol. 97:739-750.
Bhattacharyya et al., 1995, "Features of the hmg 1 subfamily of genes encoding HMG-CoA reductase in potato", Plant Mol. Biol. 28:1-15.
Bianchini et al., 1993, "Post-translational regulation of potato 3-Hydroxy-3-Methylglutaryl coenzyme A reductase", Plant Physiol. 102:(1 Suppl) 146. abstr. 834.
Bohlmann et al., 1988, "Leaf-specific thionins of barley--a novel class of cell wall proteins toxic to plant-pathogenic fungi and possibly involved in the defence mechanism of plants",EMBO J. 7:1559-1565.
Bowler et al., 1989, "The induction of manganese superoxide dismutase in response to stress in Nicotiana plumbaginifolia", EMBO J. 8:31-38.
Burnett et al., 1993, "Expression of a 3-Hydroxy-3-Methylglutaryl coenzyme A reductase gene from Camptotheca acuminata is differentially regulated by wounding and methyl jasmonate", Plant Physiol. 103:41-48.
Casacuberta et al., 1991, "A gene coding for a basic pathogenesis-related (PR-like) protein from Zea mays. Molecular cloning and induction by a fungus (Fusarium moniliforme) in germinating maize seeds", Plant Mol. Biol. 16:527-536.
Chandler & Robertson, 1994, "Gene expression regulated by abscisic acid and its relation to stress tolerance", Annu. Rev. Plant Physiol. Plant Mol. Biol. 45:113-141.
Cornelissen et al., 1986, "Molecular characterization of messenger RNAs for `pathogenesis-related` proteins 1a, 1b and 1c, induced by TMV infection of tobacco", EMBO J. 5:37-40.
Cornelissen et al., 1986, "A tobacco mosaic virus-induced tobacco protein is homologous to the sweet-tasting protein thaumatin", Nature 321:531-532.
Doerner et al., 1990, "Plant defense gene promoter-reporter gene fusions in transgenic plants: Tools for identification of novel inducers", Bio/technology 8:845-848.
Dyer et al., 1989, "Wounding induces the first enzyme of the shikimate pathway in Solanaceae", Proc. Natl. Acad. Sci. USA 86:7370-7373.
Enjuto et al., 1995, "Expression of the arabidopsis HMG2 gene, encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase, is restricted to meristematic and floral tissues"Plant Cell 7:517-527.
Gubler & Jacobsen, 1992, "Gibberellin-responsive elements in the promoter of a barley high-pl .alpha.-amylase gene", Plant Cell 4:1435-1441.
Hain et al., 1993, "Disease resistance results from foreign phytoalexin expression in a novel plant", Nature 361:153-156.
Hedrick et al., 1988, "Chitinase cDNA cloning and mRNA induction by fungal elicitor, wounding, and infection", Plant Physiol. 86:182-186.
Ho & Sachs, 1989, "Stress-induced proteins: Characterization and the regulation of their synthesis", Biochem. of Plants 15:347-378.
Jaeck et al., 1992, "Regulation of enzymes involved in lignin biosynthesis: Induction of O-methyltransferase mRNAs during the hypersensitive reaction of tobacco to tobacco mosaic virus", Mol. Plant-Microbe Interactions 5:294-300.
Keil et al., 1989, "Both wound-inducible and tuber-specific expression are mediated by the promoter of a single member of the potato proteinase inhibitor II gene family", EMBO J. 8:1323-1330.
Kononowicz et al., 1992, "Regulation of the osmotin gene promoter", Plant Cell 4:513-524.
Korth et al., 1993, "Transcriptional regulation of genes encoding HMG-CoA reductase in potato", Plant Physiol. 102:(1 Suppl)71, abstr. 390.
Lagrimini et al., 1987, "Molecular cloning of complementary DNA encoding the lignin-forming peroxidase from tobacco: Molecular analysis and tissue-specific expression", Proc. Natl. Acad. Sci. USA 84:7542-7546.
Leah et al., 1991, "Biochemical and molecular characterization of three barley seed proteins with antifungal properties", J. Mol. Biol. 266:1564-1573.
Legr
Cramer Carole Lyn
Weissenborn Deborah Louise
McElwain Elizabeth
Virginia Tech Intellectual Properties Inc.
LandOfFree
HMG2 promoter expression system and post-harvest production of g does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with HMG2 promoter expression system and post-harvest production of g, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and HMG2 promoter expression system and post-harvest production of g will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1938312