Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-06-06
1998-07-07
Horlick, Kenneth R.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
536 235, 536 243, 536 2431, 536 2433, C12Q 168, C07H 2104
Patent
active
057766762
ABSTRACT:
A full length cDNA clone from a normal human mammary epithelial cell (strain 184) encoding a 25 kDa protein, HME1, was isolated. Expression of HME1 RNA appears to be limited to epithelial cells. The HME1 sequence shares strong sequence homology with bovine 14-3-3 protein, which is an activator of tyrosine and tryptophan hydroxylase, but the tissue distribution of HME1 differs from that of 14-3-3. Compared with normal mammary epithelial cells, expression of HME1 RNA is dramatically low in cells derived from human mammary carcinoma and in normal mammary epithelial cells transformed by oncogenes. HME1 therefore appears to be a cellular differentiation marker that is down-regulated during neoplastic transformation.
REFERENCES:
patent: 4707438 (1987-11-01), Keydar
patent: 5597719 (1997-01-01), Freed et al.
Badley, et al. A simple, rapid method for the purification of poly A+ RNA. Biotechniques. 6: 114-116. (1988).
Ceriani, R., et al. Circulating human mammary epithelial antigens in breast cancer. Proc. Natl. Acad. Sci. USA 79:5420-5424. (1982).
Chou, P.Y. and G.D. Fasman. Prediction of protein confirmation. Biochemistry 13: 222-245. (1974).
Clark, R. et al. Transformation of human mammary epithelial cells by oncogenic retroviruses. Cancer Res. 48: 4689-4692. (1988).
Cooper, H.L. et al. Post-translational formation of hypusine in a single major protein occurs generally in growing cells and is associated with activation of lymphocyte growth. Cell 29:791-797. (1982).
Fuchs, E., Keratins as biochemical markers of epithelial differentiation. Trends Gen. 4: 277-281. (1988).
Hammond, S.L. et al. Serum-free growth of human mammary epithelial cells: rapid clonal growth in defined medium and extended serial passage with pituitary extract. Proc. Nat. Acad. Sci. U.S.A. 81:5435-5439. (1984).
Huse, W. et al. Generation of a large combinatorial library of the immunoglobulin repetoire in phage lambda. Science 246:1275. (1989).
Ichimura, T. et al. Molecular cloning of cDNA coding from brain specific 14-3-3 protein, a protein kinase-dependent activator of tyrosine and tryptophan hydroxylases. Proc. Nat. Acad. Sci. USA 85:7084-7088. (1988).
Imrie, S.F. et al. Detailed investigation of the diagnostic value in tumor histopathology of ICR2, a new monoclonal antibody to epithelial membrane antigen. Histopathology 16: 573-581. (1990).
Kohler, G. and C. Milstein. Continuous culture of fused cells secreting antibody of predefined specificity. Nature (London) 256:495-497. (1975).
Kozak, M. Structural features in eukaryotic mRNAs that modulate the initiation of translation. J. Biol. Chem. 266:19867-19870. (1991).
Laemmli, U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. London. 227:680-685. (1970).
Maniatis, T., E.F. Fritsch and J. Sambrook. Molecular Cloning, 2nd Edition. Cold Spring Harbor N.Y., Cold Spring Harbor Laboratory. (1989).
Merrick, W. Meth. Enzymol. 101:38. (1983).
Mullis, K., and Faloona, F. Meth. Enzymol. 155:335. (1987).
O'Brian, C.A. et al. Elevated Protein Kinase C expression in human breast tumor biopsies relative to normal breast tissue. Cancer Res. 49:3215-3217. (1989).
Patten, B.M. et al. Foundations of embryology, 3rd Ed., McGraw Hill, N.Y. (1974).
Pinkus, G.S. and P.J. Kurtin. Epithelial membrane antigen-a diagnostic discriminant in surgical pathology. Human Pathology 16: 929-940. (1985).
Prasad, G.L. et al. A cDNA encoding a muscle-type tropomyosin cloned from a human epithelial cell line: identity with human fibroblast tropomyosin TM1. Biochem. Biophys. Res. Commun. 177:1068-1075. (1991).
Rosenfeld, G.C. et al. Cloning, distribution and expressing of rat 14-3-3 protein. FASEB J. 5:A834. (1991).
Sanger, F. et al. DNA sequencing with chain-terminating inhibitors. Proc. Nat. Acad. Sci. U.S.A. 75:5463-5467. (1977).
Sasaki, M. et al. Breast cancer markers: comparison between sialyltransferase and human mammary epithelial antigens (HME-Ags) for the detection of human breast tumors grafted in nude mice. Breast Cancer Res. and Treatment 5:51-56. (1985).
Schejter, E.D. and B.Z. Shilo. The drosophila EGF receptor homolog. DER. gene is allelic to faint little ball, a locus essential for embryonic development. Cell 56:1093-1104. (1989).
Schejter, E.D. et al. Alternative 5' exons and tissue specific expression of Drosophila EGF receptor homolog transcripts. Cell 46:1091-1101. (1986).
Shibuya, M. et al. Nucleotide sequence and expression of a novel human receptor-type tyrosine kinase gene. flt. closely related to the fms family. Oncogene 5:519-524. (1990).
Soule, H.D. et al. Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50: 6075-6086. (1990).
Stampfer, M.R. Isolation and growth of human mammary epithelial cells. J. Tissue Culture Meth. 9: 107-115. (1985).
Stampfer, M.R. and J.C. Bartley. Induction of transformation and continuous cell lines from normal human mammary epithelial cells after exposure to
Toker, A. et al. Protein Kinase C inhibitor proteins: Purification from sheep brain and sequence similarity to lipocortins and 14-3-3 protein. Eur. J. Biochem. 191: 421-429. (1990).
Prasad, et al., (1991), FASEBJ, 5(6), A1650, No. 7432.
Nielson (1991), Biochem. Biophys. Acta. 1088, 425-428.
Zupan, et al. (1992), J. Biol. Chem. 267(13), 8707-8710.
Cooper Herbert L.
Prasad Gaddamanugu L.
Horlick Kenneth R.
The United States of America as represented by the Department of
LandOfFree
HME1 nucleic acids and probes does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with HME1 nucleic acids and probes, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and HME1 nucleic acids and probes will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1203994