Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1995-06-07
1998-01-06
Nucker, Christine M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 71, 435 772, 435 79, 435 792, 435 794, 435794, 435975, 5303883, 53038835, C12Q 170
Patent
active
057053316
ABSTRACT:
An antigen capture method, and an antigen capture assay diagnostic kit, for detecting the presence or concentration of HIV in a biological sample without interference from antigen-antibody immune complexes is provided. The lysate of a biological sample obtained from an animal is contacted with a detectable mount of an antibody specifically reactive with the nucleocapsid p7 antigen or an immunoreactive fragment of the p7 antigen for a time and under conditions sufficient for p7 antigen contained in the lysate to form a p7-antibody complex. The presence or concentration of this p7-antibody complex is determined to detect or quantitate the presence of HIV in the biological sample. Uses of this assay and method include detecting the presence of HIV infection in an infant born to an HIV-infected mother, monitoring the progression of HIV infection, and evaluating the effectiveness of an anti-HIV treatment administered to an animal, such as a human. Purified antibodies specifically reactive with an immunoreactive epitope specific to p7 or an immunoreactive fragment of p7 are also provided as well as an antigen capture method for detecting the presence of a lentivirus in a biological sample involving the nucleocapsid protein of the lentivirus.
REFERENCES:
patent: 5462852 (1995-10-01), Arthur et al.
Louis E. Henderson et al., "Gag Proteins of the Highly Replicative MN Strain of Human Immunodeficiency Virus Type 1: Posttranslational Modifications, Proteolytic Processings, and Complete Amino Acid Sequences," Journal of Virology, 66(4):1856-1865 (Apr. 1992).
Sharon A. Cassol et al., "Diagnosis of Vertical HIV-1 Transmission Using the Polymerase Chain Reaction and Dried Blood Spot Specimens," Journal of Acquired Immune Deficiency Syndromes, 5:113-119 (1992).
Judith N. Peisen, "Development and Application of Immunoassays to Detect and Characterize p7 Immunogenicity in HIV-1 Infected Populations," Master Thesis in Biomedical Science in the Graduate School of Hood College, pp. 17-25, 96-117 (Nov. 16, 1991).
Maadhava Ellaurie et al., "Correlation of Serum Antigen and Antibody Concentration with Clinical Features in HIV Infection," Archives of Disease in Childhood, 66(2):200-203 (Feb. 1991).
Robert J. Gorelick et al., "Noninfectious Human Immunodeficiency Virus Type 1 Mutants Deficient in Genomic RNA," Journal of Virology, 64(7):3207-3211 (Jul. 1990).
Arthur Larry O.
Henderson Louis E.
Nucker Christine M.
Stucker Jeffrey
The United States of America as represented by the Secretary Dep
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