Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2007-05-15
2007-05-15
Shukla, Ram R. (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091100, C435S091200, C536S023100, C536S023700, C536S024100, C536S024300, C536S024320
Reexamination Certificate
active
10718955
ABSTRACT:
The present invention describesHistoplasmosis capsulatumchitin synthase nucleic acid and protein sequences as reagents for the detection ofH. capsulatuminfection. Specifically, the invention describes intron sequences from theH. capsulatumchitin synthase gene which can be used for hybridization-based and PCR-based detection ofH. capsulatuminfection. In another embodiment, assays forH. capsulatumchitin synthase 2 polypeptide and/or mRNA used as a diagnostic test forH. capsulatuminfection and/or histoplasmosis. Also described is the differentiation ofH. capsulatumfromBlastomyces dermititidisbased on detection of intron 1 sequences specific toH. capsulatumchitin synthase 2. The present invention also comprises the production ofH. capsulatumstrains lacking functional chitin synthase 2 as a means to produceH. capsulatumhaving reduced pathogenicity.
REFERENCES:
patent: 4946777 (1990-08-01), Laméris et al.
patent: 5242800 (1993-09-01), Jimenez et al.
patent: 5324632 (1994-06-01), Weisberg et al.
patent: 5352579 (1994-10-01), Milliman
patent: 5360732 (1994-11-01), Berka et al.
patent: 5360901 (1994-11-01), Berka et al.
patent: 5580971 (1996-12-01), Mitsuhashi
patent: 5693501 (1997-12-01), Lee et al.
patent: 5707802 (1998-01-01), Sandhu et al.
patent: 5763169 (1998-06-01), Sandhu et al.
patent: 5919617 (1999-07-01), Bhattacharjee et al.
patent: 5994066 (1999-11-01), Bergeron et al.
patent: 5997912 (1999-12-01), Schlesinger et al.
patent: 6001564 (1999-12-01), Bergeron et al.
patent: 6117641 (2000-09-01), Berlin et al.
patent: 6180339 (2001-01-01), Sandhu et al.
patent: 6228575 (2001-05-01), Gingeras et al.
patent: 7052837 (2006-05-01), Johnson et al.
patent: 0272009 (1988-06-01), None
patent: WO 96/21741 (1996-07-01), None
patent: 804619 (1997-11-01), None
patent: WO 98/46738 (1998-10-01), None
patent: WO 99/54508 (1999-10-01), None
patent: WO 99/55874 (1999-11-01), None
Ampel, “Emerging Disease Issues and Fungal Pathogens Associated with HIV Infection,” Emerg. Infect. Dis., 2:109-116, 1996.
Altschul, S., “Basic Local Alignment Search Tool,” J. Mol. Biol., 215: 403-410, 1990.
Ausubel, F.M. et al., Short Protocols in Molecular Biology, 4thEd., Chapter 2, John Wiley & Sons, N.Y.
Beaman, B. L. et al., “Purification and Properties of a Unique Superoxide Dismutase from Nocardia Asteroides,” J. Bio. Chem., 258:91-96, 1983.
Bird, R. et al., “Single-Chain Antigen-Binding Proteins,” Science, 242:423-426, 1988.
Broaddus et al., “Bronchoalveolar Lavage and Transbronchial Biopsy for the Diagnosis of Pulmonary Infections in the Acquired Immunodeficiency Syndrom,” Ann. Intern. Med., 102(6):747-752, Jun. 1985.
Brummer et al., “Antifungal Mechanisms of Activated Murine Bronchoalveolar or Perotoneal Macrophages forHistoplasma capsulatum,” Clin. Exp. Immunol., 102(1):65-70, Oct. 1995.
Bullock, W. E. et al., “Role of the Adherence-Promoting Receptors, CR3, LFA-1, and p150,95, in Binding ofHistoplasma capsulatumby Human Macrophages,” Journal of Experimental Medicine, 165:195-210, 1987.
Castro et al., “1993 Revised Classification System for HIV Infection and Expanded Surveillance Case Definition for AIDS among Adolescents and Adults,” MMRW 41:1-14, 1992.
Chomczynski, P. et al., “Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate-Phenol-Chlorofrom Extraction,” Anal. Biochem., 162-156-159, 1987.
Church, G.M. et al., “Genomic Sequencing,” Proc. Natl. Acad. Sci., USA, 81:1991-1995, 1984.
Cockerill , F.R. et al., “Rapid-Cycle Real-Time PCR : a Revolution for Clinical Microbiology,” ASM News, 68:77-83, 2002.
Cohen et al., “Sequence of the Saccharomyces Cerevisiae CTA 1 Gene and Amino Acid Sequence of Catalase A Derived from It,” Eur. J. Biochem., 176(1):159-163, Sep. 1988.
Cohen et al., “Isolation of the Catalase A Gene of Saccharomyces Cerevisiae by Complementation of the ctal Mutation,” Mol. Gen. Genet., 200(1):74-79, 1985.
Corpet, F., “Multiple Sequence Alignment with Hierarchical Clustering,” Nucl. Acids Res. 16(22), 10881-10890, 1988.
Crowele, A. J. et al., “Evidence that Vesicles Containing Living, Virulent Mycobacterium Tuberculois or Mycobacterium Avium in Cultured Human Macrophages Are Not Acidic,” Infect. Immun., 59:1823-1831, 1991.
Davies et al., “Disseminated Histoplasmosis in Immunologically Suppressed Patients,” Am. J. Med., 64:94-100, 1978.
Deepe, G. S., “The Immune Response toHistoplasma capsulatum: Unearthing Its Secrets,” J. Lab. Clin. Med., 123:201-205, 1998.
DeGroote, M. A. et al., “Periplasmic Superoxide Dismutase Protects Salmonella from Products of Phagocyte NADPH-Oxidase and Nitric Oxide Synthase,” Proc. Natl. Acad. Sci. (USA), 94:13997-14001, 1997.
Deretic, V. et al., “Mycobacterium Tuberculosis Phagosome,” Mol. Microbiol., 31:1603-1609, 1999.
Desai et al., “Killing ofHistoplasma capsulatumby Macrophage Colony Stimulating Factor-Treated Human Monocyte-Derived Macrophages: Rols for Reactive Oxygen Intermediates,” J. Med. Microbiol., 43(3):224-229, Sep. 1995.
Eissenberg, L. G. et al., “The Interplay betweenHistoplasma capsulatumand Its Host Cells,” vol. 1, Ch. 6, W. B. Saunders Company, Ltd. London, UK, 1994.
Eissenberg, L. G. et al., “Histoplasma capulatumModulates the Acidification of Phagolysosomes,” J. Exp. Med., 177:1605-1611, 1993.
Evard et al., “The Cyanelle Genome ofCyanophora paradoxa, Unlike the Chloroplast Genome, Codes for the Ribosomal L3 Protein,” Nucleic Acid Res., 18:1115-1119, 1990.
Fang, F. C. et al., “VirulentSalmonella typhimuriumHas Two Periplasmic Cu, Zn-Superoxide Dismutases,” Proc. Natl. Acad. Sci. (USA), 96:7502-7507, 1999.
Frugoli, J. A. et al., “Catalase Is Encoded by a Multigene Family inArabidopsis thaliana(L.) Heynh,” Plant Physiol., 112:327-336, 1996.
Frugoli, J. A. et al., “Intron Loss and Gain during Evolution of the Catalase Gene Family in Angiosperms,”Genetics, 149:355-365, 1998.
Garcia, E. et al., “Molecular Characterization of KatY (Antigen 5), a Thermoregulated Chromosomally Encoded Catalase-Peroxidase ofYersinia Pestis,”J. Bacteriol., 181:3114-3122, 1999.
George et al., “Radioimmunoassay: A Sensitive Screening Test for Histoplasmosis and Blastomycosis,” Am. Rev. Respir. Dis., 124(4):407-10, Oct. 1981.
Goffeau, A. et al., “Life with 6000 Genes,” Science, 274:563-567.
Goodwin, R. A. et al., “Histoplasmosis in Normal Hosts,” Medicine (Baltimore), 60:231-266, 1981.
Goodwin, R. A., Jr. et al, “Disseminated Histoplasmosis: Clinical and Pathalogic Correlations,” Medicine, 59:1-33, 1980.
Guan, L. et al., “Molecular Evolution of Maize Catalases and Their Relationship to Other Eukaryotic and Prokaryotic Catalases,” J. Mol. Evol., 42:570-579, 1996.
Hamilton, A.J. et al., “Evidence That the M Antigen ofHistoplasma capsulatumvar. Capsulatum Is a Catalase Which Exhibits Cross-Reactivity with Other Dimorphic Fungi,” J. Med. Vet. Mycol., 28:479-485, 1990.
Hansen et al., “Functional Complementation of Catalase-Defective Peroxisomes in a Methoylotrophic Yeast by Import of the Catalase A fromSaccharomyces cerevisiae,” Eur. J. Biochem., 184(1):173-179, Sep. 1989.
Hartman, P. et al., “Immunological Defense Mechanisms in Tuberculosis and MAC-Infection,” Diag. Microbiol. Infect. Dis., 34:147-152, 1999.
Henikoff, S. et al., “Amino Acid Substitution Matrices from Protein Blocks,” Proc. Natl. Acad. Sci. USA, 89, 10915-10919, 1992.
Hochman et al., “Purification and Characterization of a Catalase-Peroxidase and a Typical Catalase from the BacteriumKlebsiella Pneumoniae,” Biochim. Biophys. Act, 1077(3):299-307, Apr. 1991.
Howard, “Studies on the Catalase ofHistoplasma capsulatum,” Infect. Immun., 39(3):1161-1166, Mar. 1983.
Howard
Johnson Clayton H.
McEwen Joan E.
Bausch Sarae
Kilpatrick & Stockton LLP
Shukla Ram R.
The Board of Trustees of the University of Arkansas
LandOfFree
Histoplasma capsulatum chitin synthase sequences and their... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Histoplasma capsulatum chitin synthase sequences and their..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Histoplasma capsulatum chitin synthase sequences and their... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3794374