Histidine kinase two-component in Candida albicans

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 691, 435 912, 4352523, 4353201, 536 232, 536 2374, 536 2432, 536 2433, C12Q 168, C12P 1934, C07H 2104, C12N 1500

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active

061209996

ABSTRACT:
The present invention relates to a histidine kinase, two-component gene (CaHK1) from Candida albicans. CaHK1 encodes a 2471 amino acid protein with an estimated molecular mass of 281.8 kDa. Also provided are vectors, host cells, antibodies and recombinant methods for producing the same. The invention further relates agonists and antagonists and to screening methods for identifying agonists and antagonists of CaHK1 polypeptide activity. The invention additionally relates to diagnostic methods for detecting CaHK1 nucleic acids, polypeptides, and antibodies in a biological sample. The present invention further relates to novel antagonists and vaccines for the prevention or attenuation of infection by Candida albicans.

REFERENCES:
Schuster, S.C. et al. EMBO Journal 15(15):3880-3889, 1996.
Nagasawa, S. et al. Mol. Microbiol. 6(6):799-807, 1992.
Hrabak, E.M. et al. J. Bacteriol. 174(9):3011-3020, May 1992.
Schuster, S.C. et al, Accession No. S71628, 1996.
Calera, J.A. et al., Yeast, vol. 14:665-674 (1998).
Bassler, B.L., et al., Molecular Microbiology, vol. 13:273-286 (1994).
Hrabak, E.M. et al., J. Bacteriology, vol. 174:3011-3020 (1992).
International Search Report (PCT/US98/14254) (1998).
Nagasawa, S. et al, Accession No. S20550, 1992.
Hrabak, E.M. et al, Accession No. B41863, 1992.

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