Hirudin fusion proteins and preparation of hirudin

Details Hirudin fusion proteins and preparation of hirudin Hirudin fusion proteins and preparation of hirudin

1994-06-20

1997-04-29

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 691, 4353201, 530300, 530350, C12P 2106, C12N 500, C07K 14815

Patent

active

056248229

ABSTRACT:
Fusion proteins composed of protein A and hirudin peptides are used to prepare hirudin peptides.

REFERENCES:
patent: 5118790 (1992-06-01), Winant et al.
patent: 5164304 (1992-11-01), Johnson et al.
patent: 5166318 (1992-11-01), Furutani et al.
Merck Index, (1983) 10th Ed. #4613.
Dodt et al. The Complete Amino Acid Sequence of Hirudin, a Thrombin Specific Inhibitor. FEBS Lett. 165 (2) 180 (1984).
Dodt et al. Expression, Secretion and Processing of Hirudin in E. coli Using the Alkaline Phosphatase Signal Sequence FEBS Lett. 202 (2) 373 (1986).
Bergmann et al. Chemical Synthesis & Expression of a Gene Coding for Hirudin Biol. Chem. Hoppe-Seyles 367: 731 (1986).
Fortkamp et al. Cloning and Expression in E. coli of a Synthetic DNA for Hirudin DNA, 5, 511 (1986).
Dersenhofer, Crystallographic Refinement and Atomic Models of a Human F.sub.c Fragment Biochemistry 20, 2361-96 (1981).
Mokg et al. Eur J. Biochem. 156:637 (1986).
Uhlen et al. 1984 J Biol Chem 259(3): 1695-1702.
Moks et al. Biochemistry vol. 26 pp. 5239-5244 (1987).

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