Highly transformable bacterial cells and methods for...

Details Highly transformable bacterial cells and methods for... Highly transformable bacterial cells and methods for...

2006-07-18

2006-07-18

Vogel, Nancy (Department: 1636)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

C435S253600, C435S252800

Reexamination Certificate

active

07078220

ABSTRACT:
The invention provided herein includes novel gram negative bacteria cells containing the Hte mutation. Other aspects of the invention include methods for rendering gram negative bacterial cells bearing the Hte region, such asE. colicells competent for DNA transformation using any of a variety of competency inducing procedures. The competent cells of the subject invention may be frozen so as to provide for prolonged storage.

REFERENCES:
patent: 6706525 (2004-03-01), Greener et al.
Hanahan et al., “Plasmid transformation ofEscherichia-coliand other bacteria,” Methods in Enzymology, 204:63-113 (1991).
Likahacheva et al., “Induction of mutants ofEscherichia-coliK-12 with increased efficiency of plasmid transformation,”Byulleten' Eksperimental 'Noi Biologii I Meditsiny, 93 (5):81-82 (1982).
Radnis et al., “Genetic transformation inStreptococcus pneumoniae: nucleotide sequence and predicted amino acid sequence ofrecP,” Journal of Bacteriology, 172 (1):3669-3674 (1990).

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