Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1991-10-25
1992-05-26
Nucker, Christine
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 6, 435 71, 435 721, 436501, C12Q 128, C12Q 168, C12Q 100, C01N 33566
Patent
active
051167344
ABSTRACT:
The present invention is directed to a composition of matter and a process for detecting the presence of an oxidative catalyst in a biological sample. The composition comprises a precipitate formed by oxidation of a chromogenic substrate in the presence of the catalyst, together with two or more co-precipitated metals. A strong signal is formed with which to detect an oxidation catalyst which is localized to a target molecule. Target molecules may be nucleic acids, antibodies or cell surface antigens.
REFERENCES:
patent: 4687736 (1987-08-01), Newman et al.
Adams, J. C., "Heavy Metal Intensification of DAB-Based HRD Reaction Product," J. Histochem. Cytochem 29(6), 775 (1981).
Hsu, et al., "Color Modification of Diaminobenzidine (DAB) Precipitation by Metallic Ions and its Application for Double Immunohistochemistry," J. Histochem. Cytochem 30(10), 1079-1082 (1982).
Larsson, L-I, "Immunocytochemical Detection Systems," Chap. 3, pp. 84-86, 105-108 in Immunocytochemistry: Theory and Practice, Larsson, L-I (auth), CRC Press (1988).
Higgs Thomas W.
Taub Floyd E.
Digene Diagnostics, Inc.
Nucker Christine
Woodward M. P.
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