Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase
Patent
1984-01-30
1987-09-15
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving transferase
435 15, 435 19, 435 25, 435 26, 435 28, C12Q 150, C12Q 144, C12Q 126, C12Q 132
Patent
active
046939717
ABSTRACT:
A highly sensitive quantitative assay method for any one component which is L-glycero-3-phosphate (G3P), dihydroxyacetone-3-phosphate (DHAP), nicotinamide adenine dinucleotide (NAD) or reduced NAD, in a specimen to be assayed, comprising causing this component in the specimen to take part in the cycling reaction ##STR1## wherein GPO is glycerophosphate oxidase and GPDH is glycerophosphate dehydrogenase, and measuring a detectable change in the reaction system. There is thus provided a novel G3P-GHAP cycling reaction using GPO, which consumes O.sub.2 and generates H.sub.2 O.sub.2 and DHAP, with a substrate of G3P, and furthermore GPDH which consumes reduced NAD and generates NAD and G3P, with a substrate of DHAP. Examples of specimens are specimens which contain any one of G3P, DHAP, NAD or reduced NAD, or which liberate or generate such a component. By proceeding at a rate of more than ten cycles per minute and measuring the amount of a detectable change in the reaction, the component in a specimen can easily and sensitively be detected with good accuracy.
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Mahler et al. (1971) Biological Chemistry 2nd Ed. p. 30.
Enzyme Nomenclature (1975) Elsevier Sci. Publ. Co., pp. 100-101.
Deck Randall E.
Toyo Jozo Kabushiki Kaisha
Warden Robert J.
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