Chemistry: analytical and immunological testing – Biological cellular material tested
Patent
1997-06-27
2000-02-15
Le, Long V.
Chemistry: analytical and immunological testing
Biological cellular material tested
436172, 422 73, 422 8205, 422 8208, 356 39, 356336, 356337, G01N 3348
Patent
active
060252014
ABSTRACT:
The present invention provides a highly sensitive and accurate method and system for the discrimination and quantification of platelets in a whole blood sample using automated hematology instruments. The method and system of the invention provide the accurate measurements of platelet dry mass and platelet component concentration in both normal blood samples and in abnormal blood samples, such as those from thrombocytopenic patients. The determination of platelet dry mass and platelet component concentration can serve to assess the activation state of platelets since activated platelets possess measurably lower component concentrations and refractive indices than do non-activated platelets. The method and system of the invention also allows the clinician or skilled practitioner to determine the age of a blood sample on the basis of the measured parameter of platelet component concentration.
REFERENCES:
patent: 3836849 (1974-09-01), Coulter et al.
patent: 4202625 (1980-05-01), Weiner et al.
patent: 4325706 (1982-04-01), Gershman et al.
patent: 4577964 (1986-03-01), Hansen, Jr.
patent: 4727020 (1988-02-01), Recktenwald
patent: 4735504 (1988-04-01), Tycko
patent: 4778593 (1988-10-01), Yamashita et al.
patent: 4915501 (1990-04-01), Steen
patent: 4987086 (1991-01-01), Brosnan et al.
patent: 5017497 (1991-05-01), Gerard de Grooth et al.
patent: 5144224 (1992-09-01), Larson
patent: 5199576 (1993-04-01), Corio et al.
patent: 5284771 (1994-02-01), Fan et al.
patent: 5350695 (1994-09-01), Colella et al.
patent: 5360739 (1994-11-01), Fan et al.
patent: 5369037 (1994-11-01), Hansen
patent: 5378633 (1995-01-01), Von Behrens et al.
patent: 5438003 (1995-08-01), Colella et al.
patent: 5510267 (1996-04-01), Marshall
patent: 5518928 (1996-05-01), Cremins et al.
patent: 5540494 (1996-07-01), Purvis, Jr. et al.
patent: 5559037 (1996-09-01), Kim et al.
patent: 5631730 (1997-05-01), Chupp et al.
S. Holme and S. Murphy, Coulter Counter and light transmission studies of platelets exposed to low temperature, ADF, EDTA and storage at 22.degree.C. J. Lab. Clin. Med. 96, 1980, pp. 480-493.
EA Trowbridge, PM Reardon, D. Hutchinson and C. Pickering. The routine measurement of platelet volume: A comparision of light scattering and aperture impedance technologies. Clin. Phys. Physiol. Meas. 6(3), 1985, pp. 221-238.
GF Bahr and E. Zeitler. The determination of the dry mass in populations of isolated particles. Lab. Invest. 14(6), 1965, pp. 217-239.
L. Corash and B. Shafer. Use of asplenic rabbits to demonstrate that platelet age and density are related. Blood 60(1), 1982, pp. 166-171.
HHK Watson and CA Ludlam. Survival of 111-indium platelet subpopulations of varying density in normal and post-splenectomized subjects. Br. J. Hematol. 62, 1986, pp. 117-124.
JF Martin, T. Shaw, J. Heggie and DG Pennington. Measurement of the density of human platelets and its relationship to volume. Br. J. Hematol. 54, 1983, pp. 337-352.
NB Grover, J. Naaman, S. Ben-Sasson and F. Doljanski. Electrical sizing of particles in suspensions. I. Theory. Biophys. J. 9, 1969, p. 1398.
J. Hurley. Sizing particles with a Coulter Counter. Biophys. J. 10, 1974, p. 74.
S. Karpatkin and A. Charmatz. Heterogeneity of human platelets. I. Metabolic and Kinetic evidence suggestive of young and old platelets. J. Clin. Invest. 48, 1969, pp. 1073-1082.
GVR Born. Observations on the changes in shape of blood platelets brought about by adenosine diphosphate. J. Physiol. 209, 1070, pp. 487-511.
GVR Born, R. Dearnley, JG Faulks and DE Sharp. Quantification of the morphological reaction of platelets to aggregating agents and of its reversal of aggregation inhibitors. J. Physiol. 280, 1978, pp. 193-212.
M. Frojmovic and R. Panjwani. Geometry of normal mammalian platelets by quantitative microscopic studies. Biophys J. 16, 1976, pp. 1071-1089.
JM Paulus. Platelet size in man. Blood 46(3), 1975, pp. 321-336.
J. Zeigler, S. Murphy and FH Gardner. Microscopic platelet size and morphology in various hematologic disorders. Blood 51(3), 1978, pp. 479-486.
M. Kraytman. Platelet size in thrombocytopenias and thrombocytosis of various origin. Blood 41(4), 1973, pp. 587-597.
JD Bessman, LJ Williams and PR Gilmer. Platelet size in health and hematologic disease. Am. J. Clin. Path. 78, 1982, pp. 150-153.
J. Levin and JD Bessman. The inverse relationship between platelet volume and platelet number. J. lab. Clin. Med. 101, 1983, pp. 295-307.
JD Bessman, LJ Williams and PR Gilmer. The inverse relationship of platelet size and count in normal subjects, and an artifact of other particles. Am. J. Clin. Path. 76, 1981, pp. 289-293.
C. Giles. The platelet count and mean platelet volume. Br. J. Hematol. 48, 1981, pp. 31-37.
GA Threatte, C. Adrados, S. Ebbe and G. Brecher. Mean platelet volume: The need for a reference method. Am. J. Clin. Path. 81, 1984, pp. 769-772.
CB Thompson, DD Diaz, PG Guinn, M. Lapins, SR Kurtz and CR Valeri. The role of anticoagulation in the measurement of platelet volumes. An. J. Clin. Path. 80, 1983, pp. 327-332.
S. Murhpy and FH Gardner. Platelet storage at 22.degree.C; Metabolic, morphologic and functional studies. J. Clin. Invest. 50, 1971, pp. 370-377.
BS Bull and MB Zucker. Changes in platelet volume produced by temperature, metabolic inhibitors, and aggregating agents. Proc. Soc. Exp. Biol. Med. 120, 1965, pp. 296-301.
JG White and W. Krivit. An ultrastructural basis for the shape changes induced in platelets by chilling. Blood 30(5), 1967, pp. 625-635.
GI Johnston, EB Pickett, RP McEver and JN George. Heterogeneity of platelet secretion in response to thrombin demonstrated by fluorescence flow cytometry. Blood 69(5), 1987, pp. 1401-1403.
JN George, EB Pickett, S. Saucerman, RP McEver, TJ Kunicki, N. Keiffer and PJ Newman. Platelet surface glycoproteins. J. Clin. Invest. 78, 1986, pp. 340-348.
DG Penington, NLY Lee, AE Roxburgh and JR McGready. Platelet density and size: The interpretation of heterogeneity. Br. J. Hematol. 34, 1976, pp. 365-376.
L. Corash, H. Tan, JR Gralnick and B. Shafer. Heterogeneity of human whole blood platelet sub-populations. I. Relationship between buoyant density, cell volume and ultrastructure. Blood 49(1), 1977, pp. 71-85.
AJ Friedhoff, JC Miller and S. Karpatkin. Heterogeniety of human platelets. VII. Platelet monoamine oxidase activity in normals and patients with autoimmune thrombocytopenic purpura and reactive thrombocytosis: Its relationship to platelet protein density. Blood 51(2), 1978, pp. 317-323.
CB Thompson, KA Eaton, SM Princiotta, CA Rushin and CR Valeri. Size dependent platelet sub-populations: Relationship of platelet volume to ultrastructure, enzymatic acitvity, and function. Br. J. Hematol. 50, 1982, pp. 509-519.
L. Corash, JL Costa, B. Shafer, JA Donlon and D. Murphy. Heterogeneity of human whole blood platelet subpopulations. III. Density-dependent differences in subcellular constituents. Blood 64(1), 1984, pp. 185-193.
CB Thompson, JA Jakubowski, PG Quinn, D. Deykin and CR Valeri. Platelet size as a determinant of platelet function. J. Lab. Clin. Med. 101, 1983, pp. 205-213.
F. Gorstein, HJ Carroll and E. Puszkin. Electrolyte concentrations, potassium flux kenetics and the metabolic dependence of potassium transport in human platelets. J. Lab. Clin. Med. 70, 1967, pp. 938-950.
S. Karpatkin. Composition of platelets. In: Hematology, 2nd Ed. McGraw-Hill, NY, pp. 1176-1178.
TC Bithell. Platelets and megakaryocytes. In: Wintrobe's Clinical Hematology, 9th Ed. vol. 1, Lea and Febiger, Philadelphia, PA, 1993, pp. 511-529.
I. Olsson, A. Dahlqvist and A. Norden. Glycogen content of leukocytes and platelets. Acta Med. Scand. 194, 1963, pp. 123-127.
P. Barkhan and MJ Silver. The lipids of human erthrocytes and platelets and their effects on thromboplastin formation. 2nd annual meeting, Amer. Soc. Hematol., St. Louis, MO, In: Blood 15, 1980, p. 428.
EE Woodside and W. Kocholaty. Carbohydrates of human and bovine platelets. Blood 16, 1960, pp. 1173-1183.
H. Braunsteiner, K. Fellinger and F. Pakesch. Structural changes in the platelets as observed by electron microscopy. Blood 9, 1954, pp. 595-601.
N. Moha
Chapman Evelyn Sabrinah
Colella Gregory M.
Hetherington Edward J.
Paseltiner Lynn
Zelmanovic David
Bayer Corporation
Le Long V.
LandOfFree
Highly sensitive, accurate, and precise automated method and dev does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Highly sensitive, accurate, and precise automated method and dev, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Highly sensitive, accurate, and precise automated method and dev will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1905120