Highly purified proteinase K

Details Highly purified proteinase K Highly purified proteinase K

2006-09-29

2010-10-26

Carlson, Karen Cochrane (Department: 1652)

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Separation or purification

C530S418000, C530S350000, C435S219000, C435S223000

Reexamination Certificate

active

07820801

ABSTRACT:
A method for producing solid proteinase K which is insoluble in water and has high purity, the method comprising the steps of adding to an aqueous solution of proteinase K ammonium sulfate in an amount of 0.1 to 0.2 M per 5 minutes up to a final amount of 1.5 to 2 M after 1 hour, thereby precipitating the proteinase K as a solid, and isolating the solid proteinase K.

REFERENCES:
patent: WO87 06939 (1987-11-01), None
patent: WO 02/064760 (2002-08-01), None
Luchi et al., “Molecular characteristics of canine cardiac myosin,” Circulation Res 16:74-82, 1965.
Betzel, C., Gourinath, S., Kumar, P., Kaur, P., Perbandt, M., Eschenburg, S., Singh, T., “Structure of a Serine Protease Proteinase K fromTritirachium album limberat 0.98 Å Resolution”, Biochemistry 2001, 40, 3080-3088.
Ebeling, W., Hennrich, N., Klockow, M., Metz, H., Orth, H., and Lang, H., “Proteinase K fromTritirachium albumLimber”, Eur. J. Biochem. 47, 91-97 (1974).
“Proteinase K”, Fermentas Catalog, Online, 2003.
Pähler, A., Banerjee, A., Dattagupta, J., Fujiwara, T., Lindner, K., Pal, G., Duck, S., Weber, G., Saenger, W., “Three-dimensional structure of fungal proteinase K reveals similarity to bacterial substilisin”, The EMBO Journal vol. 3, No. 6, pp. 1311-1314, 1984.
Wiegers, J., Hilz, H., “A New Method Using ‘Proteinase K’ to Prevent mRNA Degradation During Isolation from HeLa Cells”, Biochemical and Biophysical Research Communications, vol. 44, No. 2, 1971.
Wiegers, J., Hilz, H., “Rapid Isolation of Undegraded Polysomal RNA Without Pnenol”, FEBS Letters 23, No. 1, 1972, pp. 77-82.

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