Highly permissive cell lines for hepatitis C virus RNA...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S005000, C435S440000, C435S455000, C435S325000, C435S370000, C536S023720

Reexamination Certificate

active

07455969

ABSTRACT:
This invention relates generally to cells and cell lines that are permissive for hepatitis C virus (HCV) replication, and methods and materials for making and using them. The subject cell line referred to as Huh-7.5 has the A.T.C.C. designation number PTA-8561, having been deposited on Aug. 1, 2007.

REFERENCES:
patent: 6127116 (2000-10-01), Rice
patent: 6630343 (2003-10-01), Bartenschlager
patent: 1 267 167 (2002-12-01), None
patent: WO 01/89364 (2001-11-01), None
patent: WO 02/059321 (2002-08-01), None
Lanford et al., “Advances in Model Systems for Hepatitis C Virus Research,” Virology, vol. 293 No. 1, pp. 1-9 (Feb. 2002).
Lindenbach et al., “Cell culture-grown hepatitis C virus is infectious in vivo and can be recultured in vitro,” Proceedings of the National Academy of Sciences, USA, vol. 103 No. 10, pp. 3805-3809 (Mar. 2006).
Sheehy et al., “In vitro replication models for the hepatitis C virus,” Journal of Viral hepatitis, Col No. 1, pp. 2-10 (Jan. 2007).
Keril Blight, Highly Permissive Cell Line for Subgenomic and Genomic Hepatitis C Virus RNA Replication, Journal of Virology, Dec. 2002, vol. 76 No. 24, pp. 13001-13014.
Raymond T. Chung, Hepatitis C Virus Replication is Directly Inhibited by IFN-alpha in a Full-Length Binary Expression System, Proceedings of the National Academy of Sciences, Aug. 2001, vol. 98 No. 17, pp. 9847-9852, USA.
Ralf Bartenschlager, Replication of the Hepatitis C Virus in Cell Culture, Antiviral Research, Oct. 2003, vol. 60 No. 2, pp. 91-102, Elsevier.
Blight et al., “Efficient initiation of HCV RNA replication in cell culture”,Science290:1972-4 (2002).
Krieger et al., “Enhancement of hepatitis C virus RNA replication by cell culture-adaptive mutations”,J Virol.75:4614-24 (2001).
Murray et al., “Persistent replication of hepatitis C virus replicons expressing the beta-lactamase reporter in subpopulations of highly permissive Huh7 cells”,J Virol.77:2928-35 (2003).

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