Highly efficient dual T7/T3 promoter vector PJKF16 and dual SP6/

Details Highly efficient dual T7/T3 promoter vector PJKF16 and dual SP6/ Highly efficient dual T7/T3 promoter vector PJKF16 and dual SP6/

1986-10-17

1991-05-21

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

4353201, 435 91, 935 31, 935 17, C12N 701, C12N 912, C12N 1510, C12N 1500

Patent

active

050174886

ABSTRACT:
A dual promoter cassette which has at one end a promoter for T3 RNA polymerase which contains a downstream sequence identical to a naturally occurring T3 promoter sequence and on the other end, a promoter for a phage DNA polymerase other than the T3 RNA polymerase. The recombinant DNA plasmid which includes the promoter. The plasmid is capable of highly efficient transcription of RNA with low concentrations of ribonucleoside triphosphates.

REFERENCES:
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Pharmacia Advertisement, in Nature, vol. 317, Oct. 1985.
Adhya, S. et al., Proc. Natl Acad Sci, vol. 78, pp. 147-151, 1981.

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