Highly active xylose reductase from Neurospora crassa

Details Highly active xylose reductase from Neurospora crassa Highly active xylose reductase from Neurospora crassa

2008-06-03

2008-06-03

Prouty, Rebecca E. (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

C435S158000, C435S252200, C435S069100, C435S252800, C536S023200

Reexamination Certificate

active

07381553

ABSTRACT:
A new xylose reductase encoding gene fromNeurspora crassawas heterologously expressed inE. colias a His-tag fusion protein and subsequently purified in high yield. This xylose reductase was shown to have a high turnover rate and catalytic efficiency, high stability at room temperature, broad pH profile, and a preference of NADPH over NADH. This enzyme is utilized in production of xylitol and other sugar alcohols such as sorbitol and also in the metabolic enhancement of organisms used for fermentation of plant biomass into ethanol.

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