High yield S-nitrosylation process

Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – Synthesis of peptides

Reexamination Certificate

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C530S345000, C530S402000, C530S408000, C530S409000, C536S112000, C536S124000

Reexamination Certificate

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07488794

ABSTRACT:
A method for producing a S-nitrosylated species is provided. The method comprises: (a) providing a deoxygenated, alkaline aqueous solution comprising a thiol and a nitrite-bearing species; (b) acidifying the solution by adding acid to the solution while concurrently mixing the solution (e.g., by vigorously stirring the solution) to produce the S-nitrosylated species; and (c) isolating the S-nitrosylated species. The nitrite-bearing species can be, for example, an inorganic nitrite, such as an alkali metal nitrite, or an organic nitrite, such as an alkyl nitrite (e.g., ethyl nitrite, amyl nitrite, isobutyl nitrite or t-butyl nitrite). The thiol is preferably a thiol-containing polysaccharide, a thiol-containing lipoprotein, a thiol-containing amino acid or a thiol-containing protein, and more preferably a thiol-containing polysaccharide such as thiolated cyclodextrin. In many preferred embodiments, the S-nitrosylated species is insoluble in the acidified solution, precipitating upon formation. The S-nitrosylated species can be isolated, for example, by a process in which the precipitate is removed from the solution (e.g., by centrifugation) and the aqueous solvent remaining in the precipitate is sublimated (e.g., by freezing the precipitate and subjecting it to a vacuum). The isolated S-nitrosylated product is preferably protected from heat, light, moisture and oxygen.

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The American Heritage College Dictionary, 3rd Edition, 1993, 874-875.
Louis J. Ignarro et al., “Mechanism of Vascular Smooth Muscle Relaxation by Organic Nitrates, Nitrites, Nitroprusside and Nitric Oxide: Evidence for the involvement of S-Nitrosothiols as Active Intermediate,”Journal of Pharmacology and Experimental Therapeutics, 1981, vol. 218(3), pp. 739-749.
Elizabeth A. Kowaluk et al., “Spontaneous Liberation of Nitric Oxide Cannot Account for in Vitro Vascular Relaxation by S-Nitrosothiols,”Journal of Pharmacology and Experimental Therapeutics, 1990, vol. 255, pp. 1256-1264.
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