Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1988-02-05
1991-04-30
Martinell, James
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 692, 435 6952, 4353201, 935 48, C12P 2100, C12P 2102, C12N 1500
Patent
active
050117726
ABSTRACT:
Recombinant vectors and a method for producing a predetermined protein in a trademark microbial host are provided. The recombinant vectors are autonomously replicable expression vectors for use in B. subtilis and E. coli and express proteins therein. The vectors contain DNA sequences which code for preprolysostaphin protein secretion sequences which are necessary and sufficient for efficient secretion of the predetermined protein by transformed hosts. A gene for a predetermined protein is inserted into the vectors such that the order of genes in the vector is 5'-vector DNA-preprolysostaphin protein secretion sequence-predetermined protein sequence-vector DNA-3'. Optionally and preferably, the vectors also contain additional DNA sequences coding for staphylococcal nuclease which is a secretable detectable protein tag. A gene for a predetermined protein is inserted into such vectors such that the order of genes in the vector is 5'-vector DNA-preprolysostaphin protein secretion sequence-predetermined protein sequence-nuclease tag sequence-vector DNA-3'.
REFERENCES:
Recsei et al.; Proc. Natl. Acad. Sci. U.S.A., 84:1127, (1987).
Heinrich et al.; Mol. Gen. Genet., 209:563, (1987).
Martinell James
Public Health Research Institute of the City of N.Y.
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