High yield method of producing pure rebaudioside A

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

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C536S004100, C536S127000, C536S128000, C514S025000

Reexamination Certificate

active

07923552

ABSTRACT:
The invention provides a high throughput, high purity, high yield system and method of isolating and purifying rebaudioside A (“Reb A”), with acceptable water solubility for all commercial uses, from commercially availableStevia rebaudianastarting material. The invention also provides a means of maximizing yields of 99+% purity Reb A based on the attributes of a given batch ofSteviastarting material. The Reb A produced by the invention is water soluble, devoid of bitterness heretofore associated with rebaudioside sweeteners, non-caloric, and suitable for use as a reagent and as an ingredient in orally consumed products, e.g., as a sweetener, flavor enhancer, and flavor modifier.

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Kolb, N., et al. J. Agric. Food Chem 2001, 49, 4538-4541.
Written Opinion of the International Search Authority in PCT/US2005/037766.
International Preliminary Examination Report in PCT/US2005/037766.
Supplementary European Search Report in EP 05810517.2-2114 (PCT/US2005/037766) (5shts) mailed to EP agent on Jun. 5, 2009.
Search and Examination Report in Chilean App. No. 1092-2007 (PCT/US2005/037766) (7 shts), undated, but mailed (on information and belief) to Chilean agent in Sep. 2009 in the U.S. on Sep. 28, 2009. The addition of the NPL document amends the IDS submitted earlier today and is the only difference between the unamended IDS and the instant, amended, IDS. Applicant does not have an English translation of this report.
Supplementary European Search Report in EP 05810517.2-2114 (PCT/US2005/037766) (5 shts) mailed to EP agent on Jun. 5, 2009.

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