High voltage nanosecond pulse generator using fast recovery...

Chemistry: molecular biology and microbiology – Apparatus – Mutation or genetic engineering apparatus

Reexamination Certificate

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C435S173600, C435S461000

Reexamination Certificate

active

07901929

ABSTRACT:
A pulse generator circuit may include a diode configured to operate as an opening switch, a tank circuit in series with the diode having an admittance that is switchable from a first value to a second value that is different from the first value, and a switching system configured to cause the tank circuit to switch between the first value and the second value. The diode may saturate in less than 100 nanoseconds. A saturable core transformer may operate as a switch that controls the opening of the diode. The pulse generator may generate a plurality of pulses, each having a length of no more than 3 nanoseconds and an amplitude of at least 1 kilovolt. Electrodes may be connected to the pulse generator to deliver the plurality of pulses to biological cells.

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Office Action, dated Mar. 4, 2009 for U.S. Appl. No. 11/279,697, entitled “High Voltage Nanosecond Pulse Generator Using Fast Recovery Diodes for Cell Electro-manipulation,” filed Apr. 13, 2006.
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