High titer production of human somatomedin C

Details High titer production of human somatomedin C High titer production of human somatomedin C

1987-04-06

1992-04-14

Weimar, Elizabeth C.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 691, 4353201, 4351723, 43525233, 435 712, 536 27, 530399, 935 33, 935 38, 935 39, 935 44, 935 61, 935 43, 935 73, C12P 2102, C07K 1300, C12N 121, C12N 1503, C12N 1518

Patent

active

051047969

ABSTRACT:
A high titer fermentation process using transformed Escherichia coli cells carrying a plasmid containing DNA which codes for Somatomedin C is disclosed. Titers of about 900-1000 mg/L have been obtained using the method of the present invention; about two times the titer of prior art processes.

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Nucleic Acids Research, vol 13(6), Mar. 25, 1985, Table of Contents.
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Buell et al., 1985 Optimizing expression in E. coli of a synthetic gene encoding somatomedin-C (IGF-I), Nic. Acids Res. 13, 1923-1938.
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