High throughtput screening of potential displacer molecules

Details High throughtput screening of potential displacer molecules High throughtput screening of potential displacer molecules

2005-04-19

2005-04-19

Celsa, Bennett (Department: 1639)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C530S412000, C530S415000, C530S416000, C530S344000, C210S198200, C210S635000, C210S656000

Reexamination Certificate

active

06881540

ABSTRACT:
An effective technique for the high throughput screening of displacers is described. In this technique, potential displacers are employed to displace a biomolecule (e.g., protein) adsorbed on a chromatographic resin in small-scale batch displacement experiments. The amount of protein displaced from a specific resin by a defined concentration of displacer is determined by monitoring the supermatant for the protein. By evaluating the displaced protein rather than the displacer itself, this technique enables a single detection technique (e.g., absorbance, fluorescence, etc.) to be employed for all batch displacement experiments. By monitoring the amount of protein displaced, the effacy of a large number of potential displacers can be rapidly evaluated. The entire experimental procedure can be carried out rapidly and is thus amenable to high throughput parallel screening of molecules possessing a large range of affinities and physico-chemical properties. The error of the technique is within 5% of protein displaced, thus making it a very reliable technique. The technique can be extended to different stationary phase materials, biomolecules, and modes of interaction.

REFERENCES:
patent: 5478924 (1995-12-01), Cramer et al.
patent: 5606033 (1997-02-01), Cramer et al.
patent: 6066848 (2000-05-01), Kassel et al.
Jayaraman, G.; Yu-Fei, L.; Moore, J. A.; Cramer, S. M. “Ion-exchange displacment chromatography of proteins. Dendritic polymers as novel displacers” Journal of Chromatography A 1995 702(1+2), 143-55.

Affiliated with

Also associated with

Rating

High throughtput screening of potential displacer molecules does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with High throughtput screening of potential displacer molecules, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and High throughtput screening of potential displacer molecules will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3372432