High throughput thermal cycler

Heat exchange – With timer – programmer – time delay – or condition responsive... – Having heating and cooling capability

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422 67, 422109, 422116, 165 64, 165168, 4352851, 4352861, 4352884, 935 87, 935 88, F25B 2900

Patent

active

056011415

ABSTRACT:
A batch thermal cycler for large numbers of biological or chemical samples uses n modules each in good thermal contact with the samples, but substantially isolated from one another, thermally and functionally. Each module carries samples on an upper sample plate. The module has a temperature sensor adjacent the samples, an electrical resistance heating element, and a circulating fluid heat exchanger for step cooling. Heating occurs at a point generally between the samples and the source of the cooling. The modules are individually replaceable. O-rings automatically seal fluid and electrical interfaces. An electrical controller has n simultaneous channels that provide closed loop control of the electrical power to each module. As a method, the invention includes at least one modular temperature zone where the temperature is sensed at a point adjacent the samples in that zone. The samples are heated adjacent the sample plate. Cooling is by a step change. The cooling overshoots a set lower temperature. A small, well-controlled heating corrects the overshoot.

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