High throughput screening for novel Bioactivities

Details High throughput screening for novel Bioactivities High throughput screening for novel Bioactivities

2005-03-29

2005-03-29

Ketter, James (Department: 1636)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S252330, C435S252350, C435S471000, C435S476000, C435S484000, C435S486000

Reexamination Certificate

active

06872526

ABSTRACT:
Disclosed is a process for identifying clones having a specified activity of interest, which process comprises (i) generating one or more expression libraries derived from nucleic acid directly isolated from the environment; and (ii) screening said libraries utilizing a fluorescence activated cell sorter to identify said clones. More particularly, this is a process for identifying clones having a specified activity of interest by (i) generating one or more expression libraries derived from nucleic acid directly or indirectly isolated from the environment; (ii) exposing said libraries to a particular substrate or substrates of interest; and (iii) screening said exposed libraries utilizing a fluorescence activated cell sorter to identify clones which react with the substrate or substrates. Also provided is a process for identifying clones having a specified activity of interest by (i) generating one or more expression libraries derived from nucleic acid directly or indirectly isolated from the environment; and (ii) screening said exposed libraries utilizing an assay requiring co-encapsulation, a binding event or the covalent modification of a target, and a fluorescence activated cell sorter to identify positive clones.

REFERENCES:
patent: 4399219 (1983-08-01), Weaver
patent: 4643968 (1987-02-01), Weaver
patent: 4647536 (1987-03-01), Mosbach et al.
patent: 4916060 (1990-04-01), Weaver
patent: 4959301 (1990-09-01), Weaver et al.
patent: 5055390 (1991-10-01), Weaver et al.
patent: 5225332 (1993-07-01), Weaver et al.
patent: 5824485 (1998-10-01), Thompson et al.
patent: 5981200 (1999-11-01), Tsien et al.
patent: 6057103 (2000-05-01), Short
patent: 6391576 (2002-05-01), Tsuchida
patent: WO 9634112 (1996-10-01), None
patent: WO 9704077 (1997-02-01), None
patent: WO 9720078 (1997-06-01), None
patent: WO 9728261 (1997-08-01), None
patent: WO 9748717 (1997-12-01), None
patent: WO 9856904 (1998-12-01), None
patent: WO 9949315 (1999-09-01), None
patent: WO9954494 (1999-10-01), None
Loader et al. (1996) Accession No. CAA61111. Obtained from the Internet on Nov. 14, 2002. <http://www.ncbi.nlm.gov/entrez/viewer.fcgi?val=CAA61111.1>.*
Davies et al (1993) in “New Crops”, Jancik and Simon, eds. Wiley, New York, pp. 176-181. Obtained from the Internet on Nov. 14, 2002. <http://www.hort.purdue.edu
ewcrop/proceedings1993/v2-176.html>.*
Plovins et al (1994) App. Environ. Microbiology 60 :4638-4641.*
Zhang et al (1991) FASEB J. 5 :3108-3113.*
Roessner et al., “Fluorescence-Based Method for Selection of Recombinant Plasmids,”BioTechniques, 19:760-764, Nov. 1995.
Wittrup et al., “Microencapsulation Selection for Isolation of Yeast Mutants with Increased Secretion ofAspergillus awamoriGlucoamylase,”Biotechnology and Bioengineering, 42(3):351-356, Jul. 1993.

Affiliated with

Also associated with

Rating

High throughput screening for novel Bioactivities does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with High throughput screening for novel Bioactivities, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and High throughput screening for novel Bioactivities will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3400343