High-throughput screening diagnostic and/or dosage method of...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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Details

C435S007200, C435S007920, C530S350000

Reexamination Certificate

active

06872538

ABSTRACT:
The present invention is related to a diagnostic an/or dosage method of an agonist and/or an antagonist and/or a modulator for a calcium-coupled receptor and/or channel and/or any other calcium-coupled protein, comprising the following successive steps: disposing the agonist and/or the antagonist and/or the modulator upon a solid support; Incubating one or more cell(s) expressing apoaequorin and said calcium-coupled receptor with coelenterazine in order to reconstitute an active aequorin in said cell(s). The present invention is also related to the diagnostic and/or dosage device intended for the method according to the invention.

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patent: 0341477 (1989-04-01), None
Shimomura, et al, 1993, Biochem. J., 296: 549-551.*
Stables, et al, 1997, Anal. Biochem., 252: 115-126.*
Le Poul, et al, 2002, J. Biomolecular Screening, 7(1): 57-65.*
Knight, et al, 1993, J. Cell Biol., 121(1): 83-90.*
Saran, et al, 1994, FEBS Letters, 337: 43-47.

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