Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase
Patent
1999-09-17
2000-11-14
Leary, Louise N.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving transferase
435 4, 530329, 530300, 530200, C12Q 100, C12Q 148, A61K 3804
Patent
active
061468428
ABSTRACT:
A high throughput enzyme screen has been developed which relies on metal chelate interaction for capture of the product of the enzymatic reaction. In the present assay system, a detectable moiety is attached to a substrate having a chelating capturable moiety, which can be captured by an immobilized metal. Detection is effected due to the presence of a detectable label on the reaction product immobilized on the solid phase. Only signal associated with tagged protein bound to the solid phase is detected. The present assay can reliably measure enzyme activity, and has high reproducibility, which benefits high throughput screening.
REFERENCES:
Burger and Still: "Synthetic Ionophores. Encoded Combinatorial Libraries of Cyclen-based Receptors for Cu .sup.2+ and Cu .sup.2+ ", J. Org. Chem. 60:7382-7383 (1995).
Casey, J. Patrick: "Biochemistry of Protein Prenylation", Journal of Lipid Research 33: 1731-1740 (1992).
Casey et al., "Enzymatic Modification of Proteins With a Geranylgeranyl Isoprenoid", Proc. Natl. Acad. Sci. USA, 88:8631-8635, (Oct. 1991).
Clarck Steven: "Protein Isoprenylation and Methylation at Carboxyl-Terminal Cysteine Residues", Ann. Rev. Biochem. 61:355-386 (1992).
Farnsworth et al., "Identification of Geralnylgeralnyl-Modified Proteins in Hela Cells", Science 247:320-322(Jan. 19, 1990).
Francis et al., "Combinatorial Approach to the Discovery of Novel Coordination Complexes", J. Am. Chem. Soc. 118:8983-8984 (1996).
Glomset et al., "Prenyl Proteins in Eukaryotic Cells: A New Type of Membrane Anchor", Trends in Biochemical Sciences, 15:139-142 (1990).
Goodman et al., "A Combinatorial Library Approach to Artificial Receptor Design", J. Am. Chem. soc. 117:11610-11611, (1995).
Horwitz et al., "Ligand Design approach for Securing Robust Oxidation Catalysts", J. Am.Chem. Soc. 120:4867-4868, (1998).
Maltese A. William: "Prosttranslational Modification of Proteins by Isoprenoids in Mammalian Cells", The Faseb Journal, 4:3319-3328(Dec. 1990).
Menard et al., "Rac1, A Low-molecular-mass GTP-binding-Protein with High Intrinsic GTPase Activity and Distinct Biochemical Properties", European Journal of Biochemistry, 206:537-546 (1992).
Moomaw and Casey ;"Mammalian Protein Geranylgeranyltransferase: Subunit Composition and Metal Requirements", The Journal of Biological Chemistry 267(24): 17438-17443 (Aug. 25, 1992.
Moores et al., "Sequence Dependence of Protein Isoprenylation", The Journal of Biological Chemistry 266(22):14603-14610 (Aug. 5, 1991).
Reiss et al., : Divalent Cation and Prenyl Pyrophosphate Specificities of the Protein Farnesyltransferase from Rat Brain, a Zinc Metalloenzyme, The Journal of Biological Chemistry 267 (9):6403-6408 (1992).
Torrado et al., "Exploiting Polypeptide Motifs for the Design of selective Cu(II) Ion Chemosensors", J. Am. Chem. Soc. 120: 609-610 (1998).
Yokoyama et al., "A Protein Geranylgeranyltransferase from Bovine Brain: Implications for Protein Prenylation specificity", Proc. Natl. Acad. Sci. USA 88: 5302-5306 (Jun. 1991).
Boisclair Michael
Josiah Serene
Halstead David P.
Leary Louise N.
Mitotix, Inc.
Vincent, Esq. Matthew P.
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