High-throughput screening assays utilizing metal-chelate capture

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase

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435 4, 530329, 530300, 530200, C12Q 100, C12Q 148, A61K 3804

Patent

active

061468428

ABSTRACT:
A high throughput enzyme screen has been developed which relies on metal chelate interaction for capture of the product of the enzymatic reaction. In the present assay system, a detectable moiety is attached to a substrate having a chelating capturable moiety, which can be captured by an immobilized metal. Detection is effected due to the presence of a detectable label on the reaction product immobilized on the solid phase. Only signal associated with tagged protein bound to the solid phase is detected. The present assay can reliably measure enzyme activity, and has high reproducibility, which benefits high throughput screening.

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