High throughput screening assays for nucleic acid ligase modulat

Details High throughput screening assays for nucleic acid ligase modulat High throughput screening assays for nucleic acid ligase modulat

1998-02-19

2000-11-28

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 435 912, C12Q 168, C12P 1934

Patent

active

06153384&

ABSTRACT:
Screening assays for identifying ligase activity modulators are provided, in both solid phase and liquid phase formats. Solid phase formats detect ligase activity by ligating a labelled nucleic acid to a capture nucleic acid in the presence of the ligase modulator and detection of the labelled nucleic acid. Liquid phase assays detect ligation-dependent changes in interactive labels between nucleic acids such as proximity quenching of fluorescent labels. Compositions, apparatus and integrated systems for assays are also provided.

REFERENCES:
patent: 5705344 (1998-01-01), Giordano et al.
Jung et al, Ultrasensitive Instrumentation for DNA Sequencing and Biochemical Diagnostics,Progress in Biomedical Optics, 2386:118-127 (1995).
Automated Column Equilbration, Washing, Sample Loading and Elution of Bench-Packed Mini-Columns, BioFeedback, 12:(3) 336-338.
Samiotaki et al., Dual Color Ligation Assay, 239-242.
Weiss et al., "Enzymatic Breakage and Joining of Deoxyribonucleic Acid", The Journal of Biological Chemistry 243:17 4543-4555 (1968).
Institoris et al., "Benzamide Potentiation of the Cytotoxicity of Bifunctional Galacticol in Resistant P388 Leukemia Correlates With Inhibition of DNA Ligase II", Cancer Chemother Pharmacol 30: 325-329 (1992).
Wang et al., "Western Blots from Sodium Dodecyl Sulfate-Polyacrylamide Gels Stained by Metal Salts", Analytical Biochemistry 180: 311-313 (1989).
Tan et al., "Natural-Product Inhibitors of Human DNA Ligase I", Biochem. J., 314: 993-1000 (1996).
Stewart Shuman, "Vaccinia Virus DNA Ligase: Specificity, Fidelity, and Inhibition", Biochemistry 34: 16138-16147 (1995).
Moore et al., "Purification and Electrophoretic Assay of T4-Induced Polynucleotide Ligase for the In Vitro Construction of Recombinant DNA Molecules", Analytical Biochemistry 75: 545-554 (1976).
John D. Karkas, "A New Method of Assay for Polynucleotide Ligase", Biochimica et Biophysica Acta 340 452-462 (1974).
Montccucco et al., "DNA Unwinding and Inhibition of T4 DNA Ligase by Anthracyclines", Nucleic Acids Research 16: 3907-3919 (1988).
Moore et al., Inhibition of Two Enzymes in De Novo Purine Nucleotide Synthesis by Triciribine Phosphate (TCN-P)*, Biochemical Pharmacology, 38:22 4045-4051 (1989).

Affiliated with

Also associated with

Rating

High throughput screening assays for nucleic acid ligase modulat does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with High throughput screening assays for nucleic acid ligase modulat, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and High throughput screening assays for nucleic acid ligase modulat will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1723772