Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2006-10-17
2006-10-17
Woitach, Joseph T. (Department: 1632)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S007210, C435S007240, C435S040500, C536S023100
Reexamination Certificate
active
07122309
ABSTRACT:
Genetic modification or selection of avians requires that large numbers of birds be genetically analyzed for sequences of interest. Typically, DNA is extracted on an individual basis from samples taken from the birds. Current methods of DNA extraction extract the DNA from blood or other tissues using tedious and time-consuming procedures. The present invention provides a high throughput screening assay for detecting a genetic sequence in multiple samples. The assay further provides a DNA extraction method that allows DNA to be extracted rapidly from multiple avian samples, such as red blood cells. The extraction method is extremely reliable and does not require that each sample be quantitated post-extraction. The extracted DNA can be used for a variety of genetic assays, including a high throughput screening assay to identify insertion of a transgene. The present invention is particularly useful for extracting DNA from nucleated RBCs. Therefore, the method can be applied towards genetic analysis of avians, fish, reptiles and amphibians.
REFERENCES:
patent: 6423488 (2002-07-01), Harvey
Campbell N.A. 1996. Biology, Fourth Edition, The Benjamin/Cummings Company, Inc., California, pp. 831-833.
Buchanan et al. Human Biology: an International Record of Research 65(4) 647-654, 1993.
“DNA Extraction from Nucleated Red Blood Cells”; J.F. Medrano, E. Aasen, L. Sharrow, BioTechniques 8 (1), 43, 1990.
“Detection of specific polymerase chain reaction product by utilizing the 5′ 3′ exonuclease activity ofThermus aquaticusDNA polymerase”; P.M. Holland, R.D. Abramson, R. Watson, D.H. Gelfand, Proc. Natl. Acad. Sci. USA 88, 7276-7280, 1991.
“Genomic DNA Microextraction: A Method to Screen Numerous Samples”; R. Ramirez-Solis, J. Rivera-Perez, J.D. Wallace, M. Wims, H. Zheng, A. Bradley, Analytical Biochemistry 201, 331-335, 1992.
“Isolation of Genomic DNA from Avian Whole Blood”; J.N. Petitte, A.E. Kegelmeyer, M.J. Kulik, BioTechniques 17 (4), 664, Jul. 14, 1994.
“Microplate DNA Preparation, PCR Screening and Cell Freezing for Gene Targeting in Embryonic Stem Cells”; G.B. Udy, M.J. Evans, BioTechniques 17 (5), 887-894, Aug. 16, 1994.
“Germline transmission of exogenous genes in chickens using helper-free ecotropic avian leukosis virus-based vectors”; P. Thoraval, M. Afanassieff, F.L. Cosset, F. Lasserre, G. Verdier, F. Coudert, G. Dambrine, Transgenic Research 4, 369-376, 1995.
“Continuous Fluorescence Monitoring of Rapid Cycle DNA Amplification”; C.T. Wittwer, M.G. Herrmann, A.A. Moss, R.P. Rasmussen, BioTechniques 22 (1), 130-138, Jan. 1997.
“Improved Protocol for Using Avian Red Blood Cells as Substrates for the Polymerase Chain Reaction”; D. Bercovich, Y. Plotsky, Y. Gruenbaum, BioTechniques 26 (6), 1080-1082, Jun. 1999.
AviGenics, Inc.
Woitach Joseph T.
Yesland Kyle D.
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