Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2006-01-31
2006-01-31
Murphy, Joseph (Department: 1646)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S173100, C435S173400, C435S288700, C324S447000, C324S692000
Reexamination Certificate
active
06991910
ABSTRACT:
A method for identifying a Na+channel blocker, including providing a cell containing a Na+channel, demonstrating both a transient and a persistent current. The cell includes a potassium (K+) channel and a Na/K ATPase (Na+pump). A fluorescent dye is disposed into the well. The fluorescent dye is sensitive to change in cell membrane potential in order to enable optical measurement of cell membrane potential. A Na+channel blocker, to be identified, is added to the well and a stimulating current is passed through the cell in an amount sufficient to generate an action potential before and after the addition of the Na+channel blocker. Thereafter, a change in cell membrane potential is optically measured.
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Adorante Joseph S.
Donello John
Ehring George R.
Allergan Inc.
German Joel B.
Murphy Joseph
Stathakis Dean G.
Voet Martin A.
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