Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2005-11-14
2010-06-22
Lucas, Zachariah (Department: 1648)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S023000
Reexamination Certificate
active
07741054
ABSTRACT:
The invention is directed to a method for performing a high throughput test to determine the presence of PrPsc in a tissue sample taken post mortem from brain, the test comprising the workflow of (A) sample preparation, (B) sample treatment, (C) sample analysis, (D) controls, and (E) classifying the results of the analysis as positive or negative. The invention is further directed to preparing a treated biological sample suspected of containing PrPsc such that it is suitable for specific detection of PrPsc, if present, the method comprising the steps of (a) homogenizing the sample; followed by (b) digesting the sample proteolytically by adding to the homogenate Proteinase K and incubating the sample; followed by (c) adding a peptidic protease inhibitor to the incubated mixture of step (b), thereby obtaining a treated biological sample suitable for specific detection of PrPsc, if present. The invention further provides a conditioned microwell plate. In addition, the invention provides a soluble fusion protein comprising one or more epitopes of prion protein as well as a carrier polypeptide.
REFERENCES:
patent: 02/093164 (2002-11-01), None
Serban et al. (1990) Neurology. 40: 110-117.
Yu and Cohen. (2004) LC-GC Europe. 2-6.
Lane et al (2003) Clinical Chemistry. 49 (10): 1774-1777.
Lee et al (1984) Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi-Abstract. 17(1): 36-47.
Ardelt, W. et al., “Turkey Ovomucoid THird Domain Inhibits Eight Different Serine Proteinases of Varied Specificity on the Same . . . Leu18-Glu19 . . . Reactive Site,” Biochemistry 1985, 24, 5313-5320.
Eberle Walter
Stock Werner
Winter Baerbel
Amick Marilyn
Horning Michelle
Lucas Zachariah
Roche Diagnostics Operations Inc.
Roche Diagnostics Operations Inc.
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